CT- B Conjugates顺行示踪显色α-晶状体蛋白对损伤后视神经纤维保护作用的实验研究  

Protective effects of α-crystallin on injured optic nerve fiber of LongEvens rats by CTB conjugates anterograde labeling

作　　者：张莉[1] 王一[1] 张辰星[1] 

机构地区：[1]第三军医大学西南医院全军眼科中心,重庆400038

出　　处：《第三军医大学学报》2007年第12期1227-1229,共3页Journal of Third Military Medical University

摘　　要：目的观察α-晶状体蛋白对损伤后视神经纤维的保护作用。方法将成年Long evens大鼠26只,其中10只用于提取α-晶状体蛋白,另外16只分成4组,分别为正常对照组和伤后2,4,6周组,每组4只。损伤神经后分别右眼经玻璃体腔注射浓度为1×10-5g/L的α-晶体蛋白5μl,左眼牛血清白蛋白5μl,分别于伤后2,4,6周进行视神经取材。取材前48h,在每只眼玻璃体腔中注射CT-B(霍乱毒素B亚单位)Conjugates2.5μl,取材固定脱水并行冰冻切片后进行激光共聚焦照相(488nm波长光)观察。结果CTB Conjugates标记的夹伤视神经中可见线状条索神经纤维染色,并呈现一定扭曲,夹杂点状或者小团块状染色。α-晶状体蛋白组的荧光强度显著高于牛血清白蛋白组。结论CTB Conjugates顺染视神经示踪技术是观察和研究损伤后视神经病理变化的有效和简便的方法,α-晶状体蛋白对损伤后的视神经纤维具有保护作用。Objective To investigate in vivo protective effects of intraocular injection of α-crystallin after optic nerve injury. Methods Sixteen rats were divided into normal control group, and 2-week, 4-week, 6- week groups after optic nerve lesion, α-crystallin and bovine serum albumin （BSA） was respectively injected into the right and left corpus vitreums after the optic nerve crush injury. CTB conjugates were injected into the vitreous of all the rats at 48 h before observing optic nerve with confocal microscope （488 nm）. Results It could be seen the normal stripe of the nerve fiber in the injuried optic nerve stained with CTB conjugates and liable and convenient method to observe the optical nerve fiber after optic nerve lesion, α-crystallin administered intraocularly at the lesion time can protect the optic nerve fiber.

关 键 词：霍乱毒素B亚单位 视神经 视神经损伤 Α-晶状体蛋白 

分 类 号：R341[医药卫生—基础医学] R446