不同浓度NO对缺氧心肌细胞损害的影响  

Effect of nitric oxide on myocardial cell injury after hypoxia in rats

作　　者：张铭[1] 黄跃生[1] 张琼[1] 

机构地区：[1]第三军医大学西南医院全军烧伤研究所,创伤、烧伤与复合伤国家重点实验室,重庆400038

出　　处：《第三军医大学学报》2007年第11期1017-1019,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金重点项目(30430680);国家杰出青年科学基金(30125040)~~

摘　　要：目的利用外源性一氧化氮（nitric oxide，NO）供体S-亚硝基谷胱甘肽（S-nitrosoglutathione，GSNO）对比观察不同浓度NO对缺氧心肌细胞活力的影响。方法培养4—6d心肌细胞分为常氧组（N）、缺氧组（H）和缺氧＋GSNO组（H＋G）；H＋G组依照加入GSNO浓度的不同又分为3组：缺氧＋GSNO30μmol／L组（H＋G30）、缺氧＋GSNO200μmol／L组（H＋G200）、缺氧＋GSNO4000μmol／L组（H＋G4000）。分别观察其对缺氧（1％O2、5％CO2和94％N2）4h心肌细胞损害的影响。结果缺氧引起培养心肌细胞上清LDH活性增加，细胞存活率（MTY法）降低，H＋G30组和H＋G4000组损伤加重，H＋G200组损伤减轻。结论一定浓度范围内的NO具有细胞保护效应。Objective To clarify the effect of NO on hypoxic myocardium in rats. Methods Myocardial cells from neonatal Wistar rats aged 1 -2 days cultured for 4 -6 days, then pretreated with GSNO （30, 200, 4 003 μmol/L） were exposed to normoxia （N） or hypoxia （H） （ 1% 02, 5% CO2 and 94% N2 ） for 4 h. The NO content and LDH in the supernatant were detected. Results NO of 1.43, 7.2 and 70 μmol/L were detected respectively in the supernatant of hypoxic myocardial cells treated by GSNO （30,200 and 4 000 μmol/L）. Compared with normoxic group, LDH activity increased and myocardial cell activity decreased markedly in H ＋ G30 and H ＋ G4000 groups but less injury was observed in H ＋ G200 group. Conclusion NO of certain concentration can markedly abate myocardial cell injury induced by hypoxia.

关 键 词：缺氧 NO S-亚硝基谷胱甘肽 保护作用 

分 类 号：R322.11[医药卫生—人体解剖和组织胚胎学] R329.24[医药卫生—基础医学]