糖尿病大鼠早期骨微结构改变及其BMP-2基因的表达  

Bone microstructure changes and bone BMP-2 expression in rats at early period of diabetes

作　　者：范伟[1] 杨妤欣[2] 陈隽[3] 蒋电明[1] 

机构地区：[1]重庆医科大学附属第一医院骨科,重庆400016 [2]重庆大学生物工程学院,重庆400044 [3]重庆医科大学附属第二医院内分泌科,重庆400010

出　　处：《第三军医大学学报》2007年第11期1053-1056,共4页Journal of Third Military Medical University

摘　　要：目的观察糖尿病大鼠早期的骨密度、骨微结构改变及骨组织中BMP-2基因的表达。方法清洁级健康成年雄性Wistar大鼠30只,按照随机数字表将其分为A组(普通饲料对照组)、B组(高脂饲料+链脲佐菌素组)和C组(普通饲料+大剂量链脲佐菌素组)。饲养12周后,分别对各组大鼠进行骨密度、光镜、扫描电镜、骨形态计量学的观察以及BMP-2基因表达等指标的检测。结果与A组相比,B组骨微结构无明显变化(P>0.05),但BMP-2基因表达增高(P<0.05),C组骨微结构明显改变(P<0.05),BMP-2基因表达降低,但差异无统计学意义(P>0.05)。结论不同的糖尿病大鼠模型早期骨微结构及BMP-2基因表达情况改变不完全一致,可能与高胰岛素血症、胰岛素抵抗有一定关系。Objective To investigate the changes pression in rats at early period of diabetes. Methods of bone density, bone microstructure, bone BMP-2 ex- Thirty SPF 4-month-old male Wistar rats were randomly divided into 3 groups （n = 10 in each group） ： A （fed with common forage as normal control group）, B （fed with high-lipid forage and intraperitoneally injected with STZ to establish type 2 diabetes with insulin resistance）, C （fed with common forage and intraperitoneally injected with STZ to establish type 2 diabetes with insulin secretion defaulting）. The bone histomorphology, bone histomorphometry, bone BMP-2 expression were investigated 12 weeks later. Results As compared with group A, bone microstructure was of no obvious changes and bone BMP-2 expression was higher in group B （ P 〈 0.05 ） , and obvious bone breakage was observed in group C （ P 〈 0.05 ）. Conclusion The changes of bone microstructure and bone BMP-2 expression at the early period of diabetes are not consistent absolutely in individual rat model of diabetes. It maybe relate to hyperinsulinism and insulin resistance.

关 键 词：糖尿病 骨微结构 BMP-2 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学] R336[医药卫生—基础医学]