银杏内酯B衍生物(XQ)对PAF、ADP等诱导血小板聚集的抑制作用  

Inhibiting Effect of Ginkolide B Derivative(XQ) on Platelet Aggregation Induced by PAF and ADP

作　　者：韦敏[1] 潘苏华[1] 沈健[1] 童黄锦[1] 许惠琴[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210046

出　　处：《南京中医药大学学报》2007年第3期179-181,共3页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省中医药局科研项目(H-024)

摘　　要：目的探索银杏内酯B衍生物(XQ)抗血小板聚集的作用及其机制。方法体内外2种途径给药,以曲克芦丁、银杏内酯B为阳性对照物,观察XQ对PAF、ADP、AA、COL诱导家兔血小板聚集的抑制作用。结果XQ体外给药对PAF、ADP、AA、COL诱导的家兔血小板聚集均有抑制作用,IC50分别为1.49×10-1、7.20×105、1.78×102、4.69×103μg/mL。XQ体内给药(剂量为0.225、0.45、1.8 mg/kg)对上述各诱导剂也均有抑制作用,与对照组比较具有显著性差异(P<0.05、0.01)。银杏内酯B体外给药对PAF、ADP、AA、COL诱导的家兔血小板聚集均有抑制作用,IC50分别为8.59×10-1、8.43×103、5.30×102、1.03×102μg/mL。曲克芦丁在体外对PAF诱导剂无作用,而体内给药(24 mg/kg)则具有抑制作用。结论XQ对PAF、ADP、AA、COL诱导的血小板聚集有抑制作用,其中对PAF诱导剂的作用强于银杏内酯B,其作用机制可能与拮抗PAF受体有关。OBJECTIVE To investigate into the inhibiting effect of ginkolide B derivative （XQ） on platelet aggregation and its mechanism. METHOD In vivo and in vitro drug administration was given with troxerutin and ginkolide B as the positive control to observe the inhibiting effect. RESULT In vitro drug administration had inhibiting effect on rabbit platelet aggregation induced by PAF, ADP, AA and COL, ICso being 1.49 ×10^-1, 7.20 × 10^3, 1.78 × 10^2 and 4.69 × 10^3μg/mL respectively. In vivo drug administration （0. 225, 0.45, 1.8 mg/kg） also had inhibiting effect, with marked difference as compared with the control group （ P 〈 0.05, 0.01 ）. These figures for in vivo drug administration were 8.59 × 10^-1, 8.3× 10^3, 30 × 10^2 and 1.03 × 10^2μg/mL respectively. Troxerutin had no effect to PAF induction with in vitro drug administration but had inhibiting effect with in vivo drug administration （24 mg/kg）. CONCLUSION XQ has inhibiting effect on platelet aggregation induced by PAF, ADP, AA and COL, with stronger effect on PAF induction than ginkolide B. Its mechanism is probably related to antagonism to PAF receptor.

关 键 词：银杏内酯B衍生物 血小板聚集 PAF诱导剂 

分 类 号：R285.5[医药卫生—中药学]