人工种植红豆杉各部位紫杉醇的提取过程分析  

Analysis of Extracting Taxol from Different Parts of Planted Yew

作　　者：回晶[1] 辛如[1] 李辉[1] 邹志远[1] 刘宏生[1] 

机构地区：[1]辽宁大学生命科学系,辽宁沈阳110036

出　　处：《辽宁大学学报（自然科学版）》2007年第2期178-180,共3页Journal of Liaoning University：Natural Sciences Edition

摘　　要：对江西人工种植红豆杉叶、根、枝中紫杉醇的提取纯化技术进行了研究,比较了加热回流与超声加热回流两种提取方法,经过固相萃取、液-液萃取、正己烷沉淀、硅胶柱层析分离纯化紫杉醇,紫外扫描检测叶、根、枝中紫杉醇的含量分别13.89μg/g、12.67μg/g、39.33μg/g,纯度分别为83%、81.7%、90%,说明红豆杉植物的枝比较适合做为提取紫杉醇的原料,并且超声加热回流法优于普通加热回流法.The extraction and purification technnologies of taxol from planted yew leaves, roots and branches are studied, and two extraction methods of ultrasonic heating and refluxing hack are compared. After solid - phase extraction and liquid - liquid extraction, precipitation of hexane, silica-gel chromatography purification of paclitaxel, ultraviolet detection is used to detect the content in the leaves, roots and baranches. They are 13.89 μg/g, 12.67 μg/g, 39.33μg/g; the purifies are 83%, 81.7%, 90%, respectively. It indicates that taxus branch is the best raw material for extracting taxol and the refluxing extraction method is better.

关 键 词：红豆杉 紫杉醇 提取 

分 类 号：Q945.31[生物学—植物学]