炎症细胞因子与实验性2型糖尿病大鼠及肾脏改变  

The changes of inflammatory factors and kidney injury in rat model of type 2 diabetes mellitus

作　　者：王战建[1] 徐焕宇[1] 苏杰英[1] 李梅轻[1] 庞建华[1] 宋庆芳[1] 王振贤[1] 张耀[1] 

机构地区：[1]河北医科大学第三医院,石家庄市050051

出　　处：《河北医药》2007年第2期99-101,共3页Hebei Medical Journal

摘　　要：探讨炎性细胞因子与实验性2型糖尿病大鼠及肾脏病变。方法通过高糖高脂饮食结合小剂量链脲佐菌素的方法建立2型糖尿病大鼠动物模型。4、8周末测量大鼠空腹血糖(FBG)、血清胰岛素(FINs),18周末测量FBG、FINs、转化生长因子β1(TGF-β1)、瘦素(Leptin)、肿瘤坏死因子(TNF-α)水平并计算胰岛素抵抗指数(HOMA-IR)。用免疫组化、反转录多聚酶链反应(RT-PCR)检测TGF-β1在肾脏的表达情况。结果2型糖尿病大鼠血清TGF-β1、TNF-α、Leptin水平明显升高,同时肾脏的TGF-β1表达水平也升高,并伴有肾脏损害。结论实验性2型糖尿病大鼠存在炎性病变及肾脏损害。Objective To investigate the changes of inflammatory factors and kidney injury in rat model of type 2 diabetes mellitus Methods The model of type 2 diabetes mellitus was set up by feeding high-glucose and highfat diet and by injecting STZ into peritoneal cavity of rat, At the end of 4 weeks and 8 weeks, the fasting blood glucose, the levels of fasting serum insulin were measured. At the end of 18 weeks the fasting blood glucose, the levels of fasting serum insulin, serum TGF-β1 ,TNF-α, Leptin in all the groups were measured. Moreover, immunohistochemistry and reverse transcription polymerase chain reaction（RT-PCR） was used to examine the pathological change of kidney and expression of TGF-β1 . Results The levels of TGF-1 ,TNF-α, Leptin in the kidney of the rats of type 2 diabetes mellitus obviously increased. The expressive levels of TGF-α in the kidney also increased. Conclusions There are inflammatory injuries in the kidney of rat model of type 2 diabetes mellitus.

关 键 词：2型糖尿病 胰岛素抵抗 炎症 转化生长因子Β1 瘦素 肿瘤坏死因子-Α 

分 类 号：R259.871[医药卫生—中西医结合]