白藜芦醇诱导人成骨肉瘤细胞凋亡及其机制  

Apoptosis induced by resveratrol in human osteosarcoma MG63 cells

作　　者：颉玉欣[1] 刘燕[2] 张进贵[1] 李亚[1] 郭晓华[1] 赵萌[1] 

机构地区：[1]河北医科大学第三医院实验中心,石家庄市050051 [2]中国医学科学院血液病研究所

出　　处：《河北医药》2007年第4期293-296,共4页Hebei Medical Journal

摘　　要：目的探讨白藜芦醇(resveratrol)诱导人成骨肉瘤细胞株MG63的凋亡作用及其分子机制。方法应用不同浓度的白藜芦醇作用于MG63细胞,利用透射电镜观察细胞形态学变化;采用四甲基偶氮唑蓝(MTT)法检测药物对细胞的增殖抑制率;流式细胞术(FCM)检测细胞凋亡、细胞周期分布;反转录聚合酶链反应(RT-PCR)检测P21cip1/WAF1 mRNA、Survivin mRNA的表达。结果白藜芦醇能明显抑制MG63细胞的增殖,并呈剂量和时间依赖性。经白藜芦醇处理MG63细胞后,透射电镜下可见到凋亡细胞,FCM分析发现,10、20μg/ml的白藜芦醇处理细胞72h后,凋亡率分别为(11.9±0.6)%、(19.7%±0.9)%(P<0.01),处理后的细胞G0/G1期比例增高,S期、G2/M期细胞比例减少;RT-PCR检测到P21cip1/WAF1表达升高,Survivin表达降低(P<0.01)。结论白藜芦醇能明显抑制MG63细胞的增殖,诱导其凋亡,其机制可能与促进P21cip1/WAF1表达、抑制Survivin的表达有关。Objective To investigate human osteosarcoma MG63 cell apoptosis induced by resveratrol and its molecular mechanism. Methods MG63 cells were treated with resveratrol of different concentrations. Transmission electron microscopy was used to observe morphological changes of cell apoptosis. MTT method was used to detect the inhibition rate . Flow cytometry was used to detect the proliferation and apoptosis of the cells . The expression of p21 mRAN and survivin mRNA were analysed by reverse transcription polymerase chain reaction （RT-PCR）.Results The cell growth was inhibited significantly, with a time and dose dependent fashion. After resveratrol treatment, MG63 cells were induced to undergo morphological changes, which exhibited small cell bulk, decreased nucleus/ cytoplasm proportion, karyopyknosis and conglomeration, which were observed by transmission electron microscopy. The subdiploid peak was observed by flow cytometry. The growth of MG63 cells was inhibited after resveratrol （10 μg/ml,20μg/ml）treatment, the inhibition rates were （11.90%±0.63%） and （19.74%±0.88%）respectively （P 〈 0.01）. The number of treated cells in G0/G1 phase increased, but that in S, G2/M phase decreased; The expression of p21^cip1/WAF1 mRNA was up-regulated, while survivin mRNA was down-regulated （ P 〈 0.01）. Conclusion Resveratrol can inhibit proliferation and induce apoptosis of MG63 cells. Its possible molecular mechanisms might be related to modulation the expression of survivin and p21^cip1/WAF1.

关 键 词：白藜芦醇 MG63 细胞凋亡 SURVIVIN p21^cip1/WAF1 

分 类 号：R977.1[医药卫生—药品]