体外抗原激活的CD4^+T淋巴细胞以TGF-β_1非依赖的途径抑制NK细胞的杀伤功能  

Stimulated CD4^+T cells in vitro inhibit cytotoxicity of NK cells in TGF-β_1 in dependent way

作　　者：王庆红[1] 王晓娟[1] 彭旭[1] 甘成军[1] 罗高兴[1] 吴军[1] 

机构地区：[1]第三军医大学西南医院全军烧伤研究所,创伤、烧伤与复合伤国家重点实验室,疾病蛋白质组,重庆市市级重点实验室,400038

出　　处：《重庆医学》2007年第10期938-939,共2页Chongqing medicine

基　　金：重庆市自然基金资助项目(STC;2006BB5071)

摘　　要：目的初步探讨体外抗原激活的CD4+T淋巴细胞对NK细胞杀伤毒性的影响及其机制。方法免疫磁珠方法获得BALB/c小鼠CD4+T淋巴细胞和NK细胞,异种皮肤抗原刺激CD4+T淋巴细胞后,与NK细胞共培养,并加入TGF-β1抗体后,分别应用51Cr标记的YAC-1检测24、48、72、96h NK细胞的杀伤毒性。结果异种皮肤抗原激活的CD4+T淋巴细胞能够持续抑制NK细胞杀伤毒性,在检测的时相点内,其抑制率分别为:39%、37.5%、26.3%和15.9%,加入TGF-β1抗体后,抑制率无明显变化。结论体外用抗原激活的的CD4+T淋巴细胞能够持续显著抑制NK细胞杀伤毒性,而且这种作用是TGF-β1非依赖的。Objective To investigate the effect and mechanism of CD4^＋ T cells stimulated with antigen acting on NK cells. Methods CD4^＋T cells and NK cells of BALB/c mice were obtained by MACS. Stimulated by heterogenous skin antigen,CD4^＋ T cells were cocultured with NK cells,moreover, the anti-TGF-β1 antibody was added into the co-culture system. The cytotoxiclty of NK cells was assayed at 24,48,72,96h by YAC-1 labeled by ^51Cr. Results The cytotoxic activity of NK cells was inhibited by antigen activated CD4^＋T cells. TGF-β1 made no sense of this action. Conclusion In vitro, CD4^＋ T cells stimulated by skin antigen suppress the function of NK cells in independent of TGF-β1.

关 键 词：CD4^+T淋巴细胞 NK细胞 TGF-Β1抗体 细胞毒性 

分 类 号：R392.12[医药卫生—免疫学]