AoGDW肽抑制血小板聚集的作用机制  

Mechanism of AoGDW inhibiting platelet aggregation

作　　者：王晓霞[1] 李晓钟[2] 刘红林[1] 解军[1] 张悦红[1] 王惠珍[1] 牛勃[1] 

机构地区：[1]山西医科大学生物化学与分子生物学教研室,山西太原030001 [2]山西省人民医院急诊科,山西太原030012

出　　处：《中国药理学通报》2007年第5期626-629,共4页Chinese Pharmacological Bulletin

基　　金：山西医科大学大学生创新基金资助项目(No200474)

摘　　要：目的研究RGD(精氨酸-甘氨酸-天门冬氨酸)肽衍生物-AoGDW(ω-氨基辛酸-甘氨酸-天门冬氨酸-色氨酸)抑制血小板聚集的作用机制。方法采用双色荧光标记以及流式细胞技术检测AoGDW肽对CD41抗体与血小板GPⅡb/Ⅲa受体结合的抑制作用以及对血小板膜上P-选择素表达的影响。结果随着AoGDW肽浓度的增加,CD41抗体与活化血小板GPⅡb/Ⅲa受体结合率呈现下降趋势,相对于阴性对照组,P<0.01。但CD41抗体与静止血小板GPⅡb/Ⅲa受体的结合率以及CD62p抗体与静止血小板P选择素的结合率与阴性对照组相比,P>0.05。结论AoG-DW肽是通过占据血小板上纤维蛋白原的结合位点即活化的GPⅡb/Ⅲa受体而发挥抑制血小板聚集的作用,并且不具有活化血小板的作用,是一种选择性的GPⅡb/Ⅲa受体拮抗剂。Aim To confirm the mechanism of AoGDW （ω-aminooctanoic acid-glycine-aspartic acid-tryptophan ） inhibiting platelet aggregation. Methods Effects of AoGDW on CD41 antibody binding to platelet GP Ⅱ b/Ⅲ a and the expression of P （ selectin were assessed by FCM （flow cytometric messurements） using two-color assay. Results AoGDW inhibited CD41 antibody binding to activated platelets in a concentration-dependent manner （ P 〈 0. 01 ） versus negative group,but did not inhibit the binding of CD41 antibody to resting platelets. P-selectin binding to CD62p was not affected by AoGDW in resting platelets. Conclusion AoGDW competitive inhibited fibrinogen binding to the activated platelet receptor glycoprotein GP Ⅱ b/Ⅲ a and had no effect on platelet activation. AoGDW peptide was a kind of inhibitor of selective platelet GP Ⅱ b/Ⅲ a receptor.

关 键 词：RGD GPⅡb/Ⅲa 流式细胞技术 作用机制 

分 类 号：R331.124[医药卫生—人体生理学] R341.6[医药卫生—基础医学]