黏附分子在大鼠骨骼肌缺血再灌注损伤中的作用  

Effect of Adhesion Molecules on Skeletal Muscle Ischemia Reperfusion Injury

作　　者：王新涛[1] 吕松岑[2] 韩竹[2] 孙宇一 闫景龙[1] 

机构地区：[1]哈尔滨医科大学附属第一医院骨科,黑龙江哈尔滨市150001 [2]哈尔滨医科大学附属第二医院骨科,黑龙江哈尔滨市150086 [3]哈尔滨市公安医院,黑龙江哈尔滨市150001

出　　处：《中国康复理论与实践》2007年第5期425-427,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：黑龙江省自然科学基金资助项目(D9827)

摘　　要：目的探讨大鼠骨骼肌缺血再灌注(I/R)时黏附分子的动态变化及其在骨骼肌I/R损伤中的作用。方法42只Wistar大鼠随机分为正常对照组(n=6)、缺血组(n=6)、缺血再灌注组(n=30)。分别于缺血期,再灌注1、2、4、8、12h测定白细胞上黏附分子β2整合素(CD11b/CD18)和骨骼肌血管中细胞间黏附分子-1(ICAM-1)的表达情况,测定血浆中的丙二醛、骨骼肌组织中的髓过氧化物酶,及各组的组织学改变。结果随着骨骼肌再灌注的时间的延长,黏附分子的表达逐渐增多,再灌注8～12h表达最多,骨骼肌组织损伤也随再灌注时间的延长而逐渐加重,时程上与黏附分子表达同步。结论黏附分子的表达与骨骼肌I/R损伤密切相关。Objective To investigate the changes of adhesion molecules and their effects on skeletal muscle ischemia/reperfusion injury. Methods 42 Wistar rats were divided into 3 groups： normal control group （Group Ⅰ , n：6）, ischemia group （Group Ⅱ , n =6） ,ischemia/reperfusion injury group （Group Ⅲ , n：30）. The level of malondialdehyde （MDA） in the plasma, myeloperoxidase （MPO） in the skeletal muscle, CD11b/CD18 on the leukocytes, intercelluar adhesion molecule-1 （ICAM-1） in the skeletal muscle and the histological changes were studied 1 h, 2 h, 4 h, 8 h, 12 h reperfusion after ischemia for 4 h. Results In group Ⅲ, the expression of CD11b/CD18 , ICAM-1 and the injury of skeletal muscle increased with the lapse of reperfusion time. They reached the peak at 8-12 hours＇ reperfusion. The injury of skeletal muscle developed with the expression of adhesion molecules. Conclusion The expression of CD11b/CD18 and ICAM-1 are significantly associated with the skeletal muscle ischemia-reperfusion injury.

关 键 词：缺血再灌注损伤 骨骼肌 Β2整合素 细胞间黏附分子-1 

分 类 号：R685[医药卫生—骨科学]