大鼠急性出血坏死性胰腺炎模型制备方法的改进  

Technique Improvement on Animal Model With Acute Hemorrhagic Necrotizing Pancreatitis in Rats

作　　者：谢荣俊[1] 张树友[1] 费书珂[2] 杨成林[1] 张春礼[1] 

机构地区：[1]南华大学附属南华医院普通外科,湖南衡阳421002 [2]南华大学第二附属医院普通外科

出　　处：《南华大学学报（医学版）》2007年第3期410-412,416,共4页Journal of Nanhua University(Medical Edition)

摘　　要：目的探讨大鼠急性出血坏死性胰腺炎模型制备方法的改进。方法36只健康SD大鼠随机分为急性出血坏死性胰腺炎组(AHNP组)18只和假手术组18只。AHNP组应用硬膜外导管经十二指肠乳头逆行胰胆管穿刺注射5%牛磺胆酸钠诱导急性出血坏死性胰腺炎;使用创面封闭胶封闭穿刺孔。两组分别于术后3、6、12 h检测腹水量、血清淀粉酶活性,观察胰腺大体及光镜下病理改变并评分。结果AHNP组胰腺呈出血坏死性改变,3、61、2 h腹水量,血清淀粉酶活性,大体及组织学评分均显著高于假手术组(P<0.05)。结论联合应用硬膜外导管和创面封闭胶对逆行胰胆管穿刺注射法加以改进,具有创伤小,操作简单,诱导成功率高,模型稳定性好等优点,是一种较理想模型制备方法。Objective To study related technique improvement on animal model with acute hemorrhagic necrotizing pancreatitis in rats. Methods The Sprague - Dawley rats were randomly divided into AHNP or Sham - operated group. Acute hemorrhagic necrotizing pancreatitis animal model was induced by injection of 5 % sodium deoxycholate through retrogradely common biliopancreatic ducts via papilla duodeni with epidural catheter, closed puncture hole with medical sealant glue. Ascites and blood sample were obtained at 3,6 and 12 hours respectively. General and histological changes of pancreas was evaluated by the corresponding score system. Results Pancreatic changes in the AHNP group was hemorrhagic and necrotizing. The ascites volume, serum amylase activity, gen- eral and histological scores in AHNP group was significantly higher than that in Sham - operated group （ P 〈 0.05 ）. Conclusion Combined epidural catheter and medical sealant glue in the experiment could minimizes the trauma and simplized the procedures. It might be a rehable model with high success rate and an ideal acute hemorrhagic necrotizing pancreatitis animal model for recommendation.

关 键 词：急性出血坏死性胰腺炎 动物模型 硬膜外导管 创面封闭胶 改进 

分 类 号：R657.51[医药卫生—外科学]