茶叶中水胺硫磷、亚胺硫磷、甲基对硫磷和伏杀硫磷农药残留的高效液相色谱法测定  

Determination of Isocarbophos,Phosemet,Methyl-parathion,Phosalone Residues in Tea Sample by High Performance Liquid Chromatography

作　　者：叶江雷[1] 弓振斌[1] 林芳[2] 金贵娥[2] 温裕云[2] 

机构地区：[1]近海海洋环境科学国家重点实验室(厦门大学) [2]厦门大学海洋与环境学院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2007年第3期441-444,共4页Journal of Xiamen University：Natural Science

基　　金：福建省自然科学基金重点项目(B2200001)资助

摘　　要：建立了茶叶中4种有机磷农药——水胺硫磷、亚胺硫磷、甲基对硫磷和伏杀硫磷残留的高效液相色谱测定方法．在45℃加温条件下用乙酸乙酯-正己烷（1：1，by vol．）混合溶剂提取待测目标物、活性炭层析柱净化，用10．0mL乙酸乙酯-正己烷（6：1，by vol．）淋洗待测组分，以Eclipse XDB-Cs色谱柱、乙腈-水混合液梯度洗脱分离、二极管阵列检测器测定．结果表明，上述4种农药在6．5min内能很好地分离；样品中添加的待测组分能定量回收，回收率89．0％～108．0％，相对标准偏差为1．89％～5．96％（1．0μg／mL，n=5），检出限分别为：水胺硫磷9．82μg／kg、亚胺硫磷7．88pg／kg、甲基对硫磷2．19pg／kg、伏杀硫磷0．72pg／kg（dm）．A method has been developed for quantitative determination of isocarbophos, phosemet,methyl-parathion,and phosalone residues in tea samples. The pesticide residues were extracted from tea samples with mixture of ethyl acetate and n-hexane （1 ： 1, by vol. ） at 45℃. The extracts were then treated with a cartridge packed with active carbon and 10 mL eluant of ethyl acetate and n-hex- ane （6 ： 1）. The 4 organophosphorus pesticides were separated with Eclipse XDB-C8 column and detected with diode array detector at programmed wavelength. The recoveries for added standard were 89.0%- 108.0 %. The relative standard deviations were 1.89 % - 5.96% （1.0 μg/mL, n=5）. The limits of quantitative determination （3a） in dry tea samples were 9.82,7.88,2. 19 and 0.72 pg/kg for isocarbophos, phosemet, methyl-parathion and phosalone, respectively.

关 键 词：茶叶 水胺硫磷 亚胺硫磷 甲基对硫磷 伏杀硫磷 高效液相色谱 

分 类 号：O657.72[理学—分析化学]