N-正辛基-N′-琥珀酰基壳聚糖的制备及其对4种肿瘤细胞的亲和性  

Preparation and research of affinity of N-octyl-N′-succinyl chitosan for four kinds of tumor cells

作　　者：许向阳[1] 周建平[1] 李玲[1] 卢是玥 杨洁[2] 

机构地区：[1]中国药科大学药剂学教研室,江苏南京210009 [2]江苏先声药物研究有限公司,江苏南京210042

出　　处：《中国新药与临床杂志》2007年第5期355-359,共5页Chinese Journal of New Drugs and Clinical Remedies

基　　金：国家自然科学基金(编号30472105)

摘　　要：目的:以不同分子质量的壳聚糖为原料合成系列的N-正辛基-N′-琥珀酰基壳聚糖,考察其与肿瘤细胞的亲和性,为其作为抗肿瘤药物的靶向载体提供依据。方法:用异硫氰酸荧光素(FITC)对N-正辛基-N′-琥珀酰基壳聚糖进行标记,再分别与人肝癌细胞(HepG2)、人白血病细胞(K562)、人非小细胞肺癌细胞(A549)及人胃癌细胞(BGC)共培养,通过流式细胞仪及酶联免疫检测仪测定N-正辛基-N′-琥珀酰基壳聚糖对肿瘤细胞的亲和性及抑制力。结果:N-正辛基-N′-琥珀酰基壳聚糖对4种肿瘤细胞亲和性明显强于空白细胞株(P＜0．01),并具有一定的肿瘤抑制作用。结论:N-正辛基-N′-琥珀酰基壳聚糖有望作为抗肿瘤药物的靶向载体。AIM： To study the affinity with tumor cells through series of N-octyl-N＇-succinyl chitosan synthesized by using different molecular weight of chitosan. METHODS： A series of N-octyl-N/-succinyl chitosan labeled with fluorescein （FITC）, were incUbated with HepG2 liver cancer cells, K562 leukemia cells, A549 human lung cancer cells, and BGC cancer cells respectively and followed by using flow cytometry and enzyme-linked immunosorbent assay to assess the affinity and inhibition of N-octyl-N＇-succinyl chitosan for the four kinds of tumor cells already mentioned. RESULTS： N-octyl-N＇-succinyl chitosan possessed stronger affinity than control cell lines and growth inhibition with the four kinds of tumor cells. CONCLUSION： N-octyl-N＇- succinyl chitosan may possibly be a tumor-targeting Carrier.

关 键 词：肿瘤细胞 培养的 酶联免疫吸附测定 壳聚糖 N-正辛基-N’-琥珀酰基亮聚祷 

分 类 号：R965[医药卫生—药理学]