耐甲氧西林金黄色葡萄球菌耐药性及其SCCmec基因型的研究  

Study on antimicrobial resistance and Staphylococcal cassette chromosome mec genotype of methicillin-resistant Staphylococcus aureus

作　　者：肖松生[1] 吴伟元 文丽霞[1] 陆坚[1] 

机构地区：[1]深圳市第三人民医院,广东深圳518020 [2]深圳市人民医院,广东深圳518020

出　　处：《中国感染控制杂志》2007年第3期153-156,共4页Chinese Journal of Infection Control

摘　　要：目的了解华南地区耐甲氧西林金黄色葡萄球菌(MRSA)的耐药性及其葡萄球菌盒染色体(SCCmec)的基因型特点。方法采用纸片扩散法检测MRSA对抗菌药物的耐药性;多重聚合酶链反应对SCCmec进行基因分型。结果MRSA的检出率为34.13%(43/126),多数菌株具有多重耐药性,但对万古霉素、氯霉素和复方磺胺甲口恶唑较为敏感。SCCmec基因型以Ⅲ型最为常见,占55.81%;其次为Ⅲa型,占25.58%;I型占6.98%;未定型占11.63%。结论华南地区MRSA具有多重耐药的特征,SCC mec基因型以Ⅲ型为主。Objective To investigate antimicrobial resistance and Staphylococcal cassette chromosome mec（SCCmec） genotype characteristics of methicillin-resistant Staphylococcus aureus （MRSA） in Southern China. Methods Antimicrobial susceptibility of MRSA was detected by Kirby-Bauer method, and SCCmec genotype by multiple PCR method. Results The incidence of MRSA was 34. 13% （43/126）, most of which were multidrug-resistant strains, but susceptible to vancomycin, chloramphenicol and trimethoprim-sulfamethoxazole. The most common SCCmec genotype （55.81%） was type Ⅲ, type Ⅲa in the second place was 25.58%, type Ⅰ 6. 98% and unidentified type 11.63%. Conclusion MRSA strains in Southern China are characterized by multidrug resistance, type Ⅲ is the most common SCCmec genotype.

关 键 词：金黄色葡萄球菌 耐甲氧西林金黄色葡萄球菌 聚合酶链反应 基因型 抗药性 微生物 

分 类 号：R378.11[医药卫生—病原生物学]