介入法建立猪急性心肌梗死再灌注模型及其效果评价  

Establishment and Evaluation of Swine model of Acute Myocardial Infarction by Interventional Technique

作　　者：胡耀鹏[1] 马根山[1] 祁春梅[1] 沈成兴[1] 陈忠[1] 张晓黎[1] 刘孝钧[1] 苏亚民[1] 李璇[1] 金琴娣[2] 

机构地区：[1]东南大学附属中大医院心血管病研究所 [2]东南大学附属中大医院放射科,江苏南京210009

出　　处：《现代生物医学进展》2007年第5期667-669,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金(30570743);东南大学国家自然科学基金预研项目(XJ0590216)

摘　　要：目的:用介入法行球囊堵闭猪冠状动脉左前降支(LAD)建立急性心肌梗死再灌注动物模型并评价其效果。方法:选用小型雄性家猪11只。麻醉后经股动脉或颈总动脉置入经皮腔内冠状动脉成形术(PTCA)球囊至冠状动脉LAD远端,堵闭血流60 min。术中持续静脉滴注胺碘酮。60min后负压撤除球囊及鞘管。行超声心动图、磁共振成像(MRI)评价心肌梗死模型建立情况,并行病理分析。结果:2只猪死于心肌梗死模型制备,存活的9只猪经超声心动图、MRI检测显示梗死部位均为左心室前壁、心尖区,部分模型累及室间隔,左室下壁,并有室壁瘤、室速等心肌梗死常见并发症的发生。结论:运用介入法经PTCA球囊LAD可成功建立猪急性心肌梗死模型。这种模型重复性强,可控性好,模型的梗死部位基本一致,易于术后评价。Objective：To investigate and develop a swine model of acute myocardial infarction （AMI） by coronary occlusion with balloon angioplasty. Methods：After anesthesia , nine swine underwent 60 minutes＇ occlusion of coronary artery. Angioplasty balloon was positioned in the mid-distal left anterior descending （LAD） . 60 minutes later, the balloon was deflated and removed. Electrocardiography and blood pressure were monitored. Echocardiography , enhanced MRI and coronary angfography were also investigated to confirm AMI. Results：AMI models were successfully induced in 9 swines. AMI were confirmed by echocardiography, enhanced MRI and further pathology. Some complications of AMI occurred during the operation. Conclusions：A closed-chest swine model of AMI is feasible and relatively effective. This model proves to be reproducible, controllable, and minimally invasive.

关 键 词：心肌梗死 动物模型 冠脉介入法 

分 类 号：R543.33[医药卫生—心血管疾病]