大鼠坐骨神经结扎后疼痛受体P2X3在背根神经节内的表达变化  

The Expression Changes of Nociceptor P2X3 in Dorsal Root Ganglia aider Sciatic Nerve Ligation

作　　者：周国兴[1] 刘求梅[1] 彭薇[1] 曾志成[2] 

机构地区：[1]湖南中医药高等专科学校形态学教研室 [2]中南大学湘雅医学院人体解剖与神经生物学系

出　　处：《现代生物医学进展》2007年第5期680-681,710,共3页Progress in Modern Biomedicine

基　　金：湖南省卫生厅资助项目(B2006-142)

摘　　要：目的:研究坐骨神经结扎损伤后疼痛受体P2X3在相应背根神经节(dorsal root ganglia,DRG)内的表达变化情况。方法:选取健康成年SD大鼠35只,建立右侧坐骨神经结扎损伤模型,采用免疫组织化学和图像分析技术检测相应L4-6DRG内P2X3的表达情况。结果:正常大鼠L4-6DRG内有大量P2X3免疫阳性神经元,坐骨神经结扎后3d P2X3表达即下调,3,7,14,21和28d其表达呈进行性下降趋势,各时间点与正常和假手术对照比较差异均有统计学意义(P＜0．05)。结论:坐骨神经结扎后P2X3在L4-6DRG内表达明显下调,提示其可能在神经源性疼痛中发挥一定的作用。Objective： To investigate the expression changes of P2X3 in dorsal root ganglia （DRG） after sciatic nerve ligation. Methods： 35 healthy adult SD rats underwent right sciatic nerve ligation. Immunohistochemistry and image analysis were used to detect the expression of P2X3 in L4-6DRG. Results：There were abundant P2X3-positive primary sensory neurons in normal L4-6DRG. The expression of P2X3 was down-regulated 3 days after sciatic nerve ligation. It also showed that a decreasing tendency appeared during the period Of 3-21 days. The expression did not lcturn to the normal level after 28 days. Conclusion：The obvious down-regulation of P2X3 expression in L4-tDRG after sciatic nerve ligation indicates that it may play an important role in neuropathic pain sensation.

关 键 词：P2X3 坐骨神经 结扎 背根神经节 神经源性疼痛 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学]