HPLC测定毛果杜鹃中金丝桃苷的含量  

Determination of hyperin in Rhododendron seniavinii Maxim.by HPLC

作　　者：谢敏[1] 周琳梅[2] 

机构地区：[1]福建省药品检验所,福州350001 [2]福建中医学院药学系,福州350003

出　　处：《海峡药学》2007年第5期35-36,共2页Strait Pharmaceutical Journal

摘　　要：目的建立高效液相色谱法测定毛果杜鹃中金丝桃苷的含量的方法。方法采用HPLC法,用C_(18)色谱柱,流动相:乙腈-0．05%磷酸(14:86),检测波长为360nm。结果金丝桃苷进样量在0．2078μg～8．312μg之间线性关系良好,金丝桃苷平均回收率为100．43%;RSD为0．9%(n=6)。结论该方法简便快速,准确实用,可用于控制毛果杜鹃的质量。OBJECTIVE To establish the method of determining hyperin in Rhododendron seniavinii Maxim. by HPLC. METHODS A HPLC method was established. HPLC conditions were as follows：Hypersil C18column, a mobile phase of acetonitrile-0. 05% phosphoric acid（ 14： 86）,detection wavelength was at 360nm. RESULTS Quercitrin has a good linear relationship between 0. 207μg -8.3120μg. And the average recoveries of quercitrin was 100.43%, RSD was 0.9% （n = 6）. CONCLUSION This method is simple, accurate. It can be used to control the quality of Rhododendron seniavinii Maxim.

关 键 词：HPLC 毛果杜鹃 金丝桃苷 

分 类 号：R927.2[医药卫生—药学]