HBsAg阳性血清HBV DNA与HBV标志物定量测定的相关性特征  

Correlation of quantitative determination of HBV DNA and HBV markers in HBsAg positive sera

作　　者：成军[1] 孙长贵[1] 王国政[1] 周乐光[2] 孙关忠[1] 

机构地区：[1]解放军第一一七医院检验科,杭州310013 [2]温州医学院检验系2005届毕业生,325000

出　　处：《国际检验医学杂志》2007年第5期385-386,389,共3页International Journal of Laboratory Medicine

基　　金：南京军区医学科研“十五”计划课题（02MB018）

摘　　要：目的分析HBsAg阳性血清HBV DNA与HBV标志物（HBV M）含量之间的量化关系,揭示两种HBVM血清学模式HBV DNA与HBV M含量的相关性特征。方法对225例血清标本采用PE7000型FQ-PCR仪和AXSYM免疫分析仪分别进行HBV DNA和HBVM的定量测定,以HBV M血清学模式进行HBV DNA分组并分析比较各组的相关性及特点。结果HBsAg/HBeAg/抗-HBc阳性模式的HBV DNA含量54.5%分布在107拷贝/ml以上,HBV DNA的C^G组及抗-HBs、HBeAg、抗-HBe的F^G组含量分别与A组比较差异有统计学意义,且HBV DNA含量与抗-HBs呈负相关（r=-0.670）、与HBeAg和抗-HBe则呈正相关（r=0.524、r=0.814）;而HB-sAg/抗-HBe/抗-HBc阳性模式的HBV DNA含量82.2%分布在105拷贝/ml以下,HBV DNA的B^G组分别与A组比较差异有统计学意义,且仅与抗-HBs呈负相关（r=-0.569）。结论不同HBV M血清学模式HBV DNA与HBV M含量之间存在不同的相关性特征。Objective To reveal the correlation of quantitative determination of HBV DNA and HBV markers in HBsAg positive sera. Methods HBV DNA and five HBV markers （including HB- sAg, anti-HBs, HBeAg, anti-HBe and anti-HBc） in 225 samples of HBsAg positive sera were quantitatively determined by using PE7000 FQ-PCR analyser and AXSYM instrument respectively. Results The HBV DNA level in 54. 5% of samples with HBsAg/HBeAg/anti-HBc positive was above 10^7copies/ml, and that of 82. 2% of samples with HBsAg/anti-HBe/anti-HBc was below 10^5 copies/ml. As compared with that of A group, the differences was significantly in C-G groups and B -G groups of HBV DNA, F-G groups of anti-HBs, HBeAg, anti-HBe. In F-G groups, the correlation coefficient was -0. 670 of HBV DNA and anti-HBs, 0. 524 of HBV DNA and HBeAg, 0. 814 of HBV DNA and anti-HBe. In BuG groups, the correlation coefficient was -0. 569 of HBV DNA and anti-HBs. Conclusion The relativity characterization is different between HBV DNA and HBV M in different serological patterns.

关 键 词：肝炎表面抗原 乙型 DNA 病毒 肝炎病毒 乙型 

分 类 号：R446.62[医药卫生—诊断学]