酶免法检测乙肝表面抗原实验精密度研究  

Study on precision of HBsAg detection with enzymoimmunoassay

作　　者：陈元锋[1] 李钦伟[1] 

机构地区：[1]山东省血液中心,济南250014

出　　处：《国际检验医学杂志》2007年第5期395-396,共2页International Journal of Laboratory Medicine

摘　　要：目的探讨酶免法检测HBsAg实验精密度的影响因素及实验精密度对检验结果的影响。方法检测卫生部临检中心0.5ng/ml质控血清,计算精密度;比较手工操作与全自动酶免分析仪法检测的精密度;检测不同效价的阳性血清,比较精密度,并作统计学分析。结果临界值附近的标本可能引起误判,手工操作与全自动酶免分析仪法检测实验精密度差异有统计学意义（t=4.72,P〈0.01）。结论实验精密度的高低对酶免法检测HBsAg实验存在影响,检验结果的判定应设置灰区并进行复检或进一步检测。Objective To probe into influential factors of HBsAg detection precision with EIA. Methods 0.5 ng/ml of quality control blood sera from the Clinical Laboratory Center of the National Ministry of Health were used as samples, and different titre positive sera were measured with fully automated EIA analyzer and manual method respectively. Results The result neighboring the cutoff value might cause the miscarriage. There was significant difference on precision of HBsAg detection between fully automated EIA analyzer and manual method （t=4.72,P〈0.01）. Conclusion The precision affects HBsAg detection by ELA. To ensure the reliability of examination result, the gray area should be established and second screening or further examination should be carried on.

关 键 词：肝炎表面抗原 乙型 酶联免疫吸附测定 

分 类 号：R446.61[医药卫生—诊断学]