溃结灵对溃疡性结肠炎大鼠治疗作用的病理学观察  

Pathological Observation of Therapeutic Action of Kuijieling Decoction on Ulcerative Colitis Rats

作　　者：杜群[1] 李红[1] 王建华[1] 王汝俊[1] 

机构地区：[1]广州中医药大学脾胃研究所,广州510405

出　　处：《中药新药与临床药理》2007年第3期173-175,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金资助项目(30400613)

摘　　要：目的观察溃结灵对实验性大鼠溃疡性结肠炎的治疗作用。方法采用三硝基苯磺酸灌肠法制作溃疡性结肠炎大鼠模型,溃结灵采用高、中、低3个剂量(18.3,9.2,4.6g/kg)给药,治疗结束时眼眶采血,酶联免疫法检测血清IL-1β含量。处死动物取结肠,肉眼观察肠黏膜的病变并按损伤程度积分,制作病理切片,HE染色,光镜下评价黏膜损伤指数。结果溃结灵高、中剂量组组织损伤积分分别为2.41±0.79和2.83±0.71,明显少于模型组(3.75±0.75)(P<0.01);光镜下观察溃结灵高、中剂量组肠黏膜病理损害程度明显轻于模型组(P<0.05或P<0.01);溃结灵高、中剂量组血清样本中IL-1β的含量明显低于模型组血清样本(P<0.01)。结论溃结灵对溃疡性结肠炎大鼠模型有较好的治疗作用,其作用机制可能与其降低血清IL-1β含量有关。Objective To observe the therapeutic effect of Kuijieling Decoction on experimental ulcerative colitis rats. Methods Ulcerative colitis rat model was induced by enteroclysis with trinitro - benzene - sulfonic acid （TNBS）. Three dosages （18.3 g/kg, 9.2 g/kg, 4.6 g/kg） of Kuijieling Decoction were used to treat the model rats. After treatment, the serum of rats were obtained to detect the level of IL - 1β by enzyme - linked immunosorhent assay. Then the rats were killed for the observation of the colonic colon mucosa. Finally, the colon tissue pathologic changes by naked eye and for the estimation of the damage scores of was fixed to make pathological section and the damage index of colon mucosa was observed under microscope. Results The tissue damage scores of high - and moderate - dosage groups of Kuifieling Decoction were （2.41 ±0. 79） and （2.83 ±0. 71） respectively, and were significantly lower than that of model control group（3.75 ± 0.75; P 〈 0.01）. Observed under microscope, the pathological damage was significantly relieved in high - and moderate - dosage Kuifieling Decoction groups as compared with that in the model control group （ P 〈 0.05 or P 〈 0.01）. The serum level of IL- 113 in high- and moderate- dosage Kuijieling Decoction groups was significantly lower than that in the model control group （ P 〈 0.01 ）. Conclusion Kuijieling Decoction has protective effect against rat ulcerative colitis, and its mechanism may be related with the reduction of IL - 1β.

关 键 词：溃结灵 溃疡性结肠炎 动物模型 白介素-1Β 酶联免疫 

分 类 号：R285.5[医药卫生—中药学]