柚皮苷对前脂肪细胞3T3-L1增殖和诱导分化的影响  

Effect of Naringin on Proliferation and Differentiation of 3T3-L1 Preadipocyte

作　　者：刘新迎[1] 周联[1] 梁瑞燕[1] 曹柳英[1] 王培训[1] 

机构地区：[1]广州中医药大学免疫研究室,广州510405

出　　处：《中药新药与临床药理》2007年第3期176-179,共4页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的观察柚皮苷对前脂肪细胞3T3-L1增殖和分化的影响,并探讨其影响3T3-L1分化的可能作用机制。方法培养3T3-L1细胞,并用不同浓度的柚皮苷进行干预,以四甲基偶氮唑盐(MTT)法检测细胞增殖,用油红O染色和染色比色法分析脂肪细胞的分化程度。逆转录多聚酶联反应(RT-PCR)检测脂肪细胞分化相关基因过氧化物酶增殖物激活受体γ2(PPARγ2)、CCAAT/增强子结合蛋白α(C/EBPα)mRNA的表达。结果柚皮苷能抑制3T3-L1前脂肪细胞的增殖和分化,并能抑制PPARγ2、C/EBPα基因的表达。结论柚皮苷可抑制3T3-L1前脂肪细胞的增殖,并能通过下调分化相关基因的表达抑制分化。Objective To observe the effect of naringin on proliferation and differentiation of 3T3 -L1 preadipocyte, and to investigate its possible mechanism. Methods The cultured 3T3 -L1 cells were pretreated with naringin, and then MTT method was used to detect the proliferation of the cells, oil red O staining method and spectrophotography were applied to analyze the degree of differentiation. The expression of PPARγ2 and C/EBPα mRNA associated with adipocytes differentiation were detected by reverse transcription PCR（RT- PCR）. Results Naringin suppressed the proliferation and differentiation of 3T3 - L1 preadipocytes and down - regulated the expression of PPARγ2 and C/EBPα mRNA. Conclusion Naringin can inhibit the proliferation of 3T3 - L1, and can suppress their differentiation through down - regulating the expression of PPARγ2 and C/EBPα mRNA.

关 键 词：柚皮苷 3T3-L1细胞 增殖分化 基因表达 

分 类 号：R285.5[医药卫生—中药学]