雷公藤多苷致雌性小鼠肾虚生殖功能低下动物模型的研究  

Establishment of Mice Model of Reproductive Hypofunction with Kidney Deficiency Caused by Tripterygium Glucosides

作　　者：谌婕[1] 吴克明[1] 王家葵[1] 熊巍[2] 

机构地区：[1]成都中医药大学,成都610075 [2]成都市医学信息所,成都610016

出　　处：《中药新药与临床药理》2007年第3期208-211,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：四川省教育厅科研基金资助项目(2004C004);四川省科技厅科研基金资助项目(04029-011-1)

摘　　要：目的探讨雷公藤多苷(GTW)致育龄期雌性小鼠生殖功能低下的药理作用机理,建立GTW致雌性小鼠生殖功能低下的动物模型。方法采用7～8周龄雌性性成熟昆明种小鼠,随机分为GTWA(60mg·kg-1·d-1)、B(40mg·kg-1·d-1)、C(30mg·kg-1·d-1)、D(20mg·kg-1·d-1)、E(15mg·kg-1·d-1)5组及正常对照组。观察给药后小鼠动情周期,分别在部分小鼠周期延长,周期均延长、不规则,周期完全紊乱、甚至消失等3个时段分批处死小鼠,考察GTW在不同时段对小鼠生殖功能的影响。采用放免法检测血清E2、P水平,计算卵巢、子宫指数,卵巢、子宫作组织形态学观察,计数各级卵泡、黄体数,测量子宫内膜、肌层厚度。结果给药10周时,A、B、C组子宫指数明显降低,子宫内膜、肌层变薄,卵巢成熟卵泡数及黄体数减少;A、B组E2水平降低,各组P水平及卵巢指数无明显变化。结论GTW连续给药10周,可损伤雌性小鼠生殖功能,造成肾虚生殖功能低下状态。建议GTW40mg·kg-1·d-1给药10周建立雌性小鼠肾虚生殖功能低下模型。Objective To study the pharmacological mechanism of glucosides of tripterygium （GTW） inducing reproductive hypofunction in female mature mice and to establish female mice model of reproductive hypofunction. Methods Female Kunming mice aged 7-8 weeks were randomly divided into 6 groups, including GTW groups of A, B, C, D and E （60, 40, 30 , 20, 15 mg· kg^-1· d^-1） and normal group. Estrous cycle of five GTW groups was observed successively. To study the effect of GTW on reproductive function in different period, three batches of mice were killed when estrous cy- cle of some mices was prolonged, estrous cycle of all the mice was prolonged or became irregular, or estrous cycle of all the mice was totally disturbed or even disappeared. Serum E2 and P levels were measured by radioimmunoassay. Ovary and uterus index were measured by tissue - weight - assay, and tectology observation on ovary and uterus was conducted. The numbers of follicle and corpus luteum as well as thickness of endometrium and myometrium were also measured. Results The mice were killed in batches after administration for 5, 7, 10 weeks. After 10 - week treatment, uterus index and the numbers of follicle and luteum were decreased significantly, and endometrium and myometrium also became obviously thinner in groups A, B, and C. E2 level in groups A and B was decreased. P level and ovary index of all treatment groups were not decreased after 10 - week treatment. Conclusion GTW at the dosages of 60, 40 and 30 mg · kg^-1· d^-1 can damage female mice＇s reproductive function and can induce mice reproductive hypofunction with kidney deficiency after 10- week successive treatment. GTW at the dosage of 40 mg · kg^-1 · d^-1 for 10 weeks is recommended to establish female mice model of reproductive hypofunction with kidney deficiency.

关 键 词：雷公藤多苷 小鼠 生殖功能低下 肾虚 动物模型 

分 类 号：R285.5[医药卫生—中药学]