适宜AFLP分析的龙眼基因组DNA提取方法  

A method for genomic DNA preparation of longan suitable to AFLP analysis

作　　者：李冬波[1] 李江舟[2] 朱建华[1] 

机构地区：[1]广西农业科学院园艺研究所,广西南宁530007 [2]广西大学农学院,广西南宁530005

出　　处：《西南农业学报》2007年第3期478-480,共3页Southwest China Journal of Agricultural Sciences

基　　金：广西自然科学基金资助项目(桂科自0339023)

摘　　要：以龙眼“石硖”、“大乌圆”、“大广眼”、“龙荔”叶片为试材,研究了一种适宜AFLP分析的龙眼基因组DNA提取方法,其改良策略是:在细胞核被裂解前先除去多酚类等次生物质,防止多酚类的氧化,细胞裂解后再加入10%的CTAB,进一步除去杂质。此方法不仅适用于嫩叶采样,也适用于老叶采样,所提取的基因组DNA可成功进行AFLP标记分析,为龙眼的分子生物学研究提供了科学依据。A method for genomic DNA preparation of longan that is suitable to AFLP analysis has been described. The modification is to remove most of the cytoplasm components before lysos of the nuclei. After lysos of the nuclei, 1/10 volume of 10% CTAB was added to remove more secondary metabolites. With the modified method ,not only young leaves but also old leaves could be used to DNA preparation. The genomic DNA samples prepared with this method could be used as templates for amplified fragments length polymorphism（ AFLP）.

关 键 词：龙眼 DNA提取 AFLP 

分 类 号：S667.2[农业科学—果树学]