婴幼儿变应原过筛试验在儿童变态反应性疾病检测中的应用  

Application of Phadiatop Infant in Detection of IgE-Mediated Allergic Diseases in Children

作　　者：赵京[1] 宋欣[1] 马煜[1] 陈育智[1] 

机构地区：[1]首都儿科研究所附属儿童医院哮喘防治教育中心,北京100020

出　　处：《国际儿科学杂志》2007年第3期162-165,共4页International Journal of Pediatrics

摘　　要：目的了解婴幼儿变应原过筛试验（phadiatop infant）作为筛查吸入物抗原及常见食物抗原血清中特异性IgE（sIgE）的工具，在儿童IgE介导的变态反应性疾病中检测的意义，并同时与吸入性变应原过筛试验（phadiatop）和儿童常见食物sIgE测定进行比较。方法临床诊断为湿疹、哮喘和变应性鼻炎等变态反应性疾病的0—12岁患儿560例，使用法玛西亚UniCAP100全自动免疫荧光系统进行血清phaditop infant和phadiatop测定。在phaditop infant筛查试验阳性的患儿中随机抽取112名进行牛奶、鸡蛋白、鱼、小麦、花生、黄豆、虾及牛肉8种常见食物的sIgE测定。结果560例患儿中328例phadiatop infant阳性，占59％；phadiatop阳性213例，占38％。所有phadiatop检测阳性的患儿phadiatop infant均阳性；而phadiatop infant检测阳性的患儿中115名phadiatop结果为阴性；小于3岁组患儿phadiatop infant检测的阳性率显著高于phadiatop检测阳性率（x^2=240．88；P〈0．001）。患儿血清phadiatop infant的IgE浓度（kU／L）与各种食物sIgE浓度之和呈正相关（r=0．67，P〈0．001），phadiatop infant和phadiatop均阳性的患儿IgE水平明显相关性（r=0．55，P〈0．001）。结论Phadiatop infant可以有效地检测血清中吸入物及食物特异性IgE；3岁以下儿童中phadiatop infant的阳性率明显高于phadiatop，可用此单一的检测除外婴幼儿和儿童非sIgE介导的相关性疾病，是体外变应原筛查的理想方法。Allergy-Like symptoms are common in young children, but the case history and physical examination cannot identify the underlying origins of overlapping symptom profiles. Objective To evaluate a blood test, Phadiatop Infant （Phannacia Diagnostics AB, Uppsala, Sweden）, in differentiating the capability of IgE-mediated diseases in children and to compare the result with that of Phadiatop and the sum slgE of several kinds of common foods.Methods Five hundred and sixty children diagnosed as eczema,asthma and rhinitis were tested by Phadiatop Infant and Phadiatop. One hundred and twelve children were selected randomly from Phadiatop Infant positive ones to test 8 kinds of contain food specific serum IgE （milk, egg white, fish, wheat,peanut, soy bean,beef and shrimp）. Results There are 328（59%） patients whose Phadiatop Infant results are positive and 213（38%） patients whose Phadiatop results are positive（ X^2 = 240.88; P 〈 0.001）in 560 patients. AU the Phadiatop Infant results of Phadatop positive children are positive but 115 Phdiatop Infant positive children whose Phadatop results are negative. Phdiatop Infant positive rate is significant higher than Phadiatop positive rate in patients younger than 3 years old（x^2 = 240. 88; P 〈 0.001 ）. There is significant positive relationship between IgE contention of Phadiatop Infant and the sum of food sIgE （r = 0.67; P 〈 0. 001 ）;and there is also obvious positive correlation with patients whose both Phadiatop Infant and Phadiatop are positive （ r = 0.55, P 〈 0. 001）. Condusions Phdiatop Infant as a single blood test has higher positive test rate in detecting IgE-mediated diseases than Phaditop,especially in children younger than 3 years old. It can detect specific IgE antibodies of food and inhalant allergens at the same time. It can be a good single screen test for allergic diseases clinically.

关 键 词：变应原 变态反应 免疫学 

分 类 号：R72[医药卫生—儿科]