病毒蛋白质组学及其应用  

The Viral Proteomics and its Applications

作　　者：朱联辉[1] 郑涛[1] 赵达生[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071

出　　处：《生物技术通讯》2007年第3期505-510,共6页Letters in Biotechnology

摘　　要：病毒蛋白质组学是蛋白质组学研究技术在病毒学领域的应用,其研究方法主要是基于质谱鉴定的电泳分离或色谱分离技术。病毒蛋白质组学的研究可以补充基因组注释、纯化单一的病毒成分、研究病毒与其宿主细胞蛋白的相互作用、识别病毒作用的靶位点、鉴定病毒感染的致病因子及病毒的进化关系、识别病毒的免疫源性蛋白。病毒蛋白质组的研究有助于对病毒致病性的了解,加速新的诊断方法及治疗药物的研制,增强对病毒的生物防御。由于一些技术及主观因素的影响,病毒蛋白质组的研究是很有限的,这是一个亟待重视并增强的领域。The viral proteomics is the application of proteomics techniques in virology, the viral proteomics method is mainly electrophoresis or liquid chromatography separation based on the mass spectrometry. In viral proteomics, we could complement the annotations of the viral genome, purify simple viral proteins, elucidate the protein interactions between virus and it host, identify the target sites virus affected, identify the agent of a viral disease and judge the evolutionary links. Viral proteomics is helpful for understanding of the pathogenicity of the viruses, increasing the development of the new diagnosis methods and medicine, reinforcing the biodefense against the viral biological warfare agent. Because of some technique problems and incorrect viewpoints, the application of viral proteomics has been limited. This is a research field needed to be thought much of and be enhanced.

关 键 词：病毒 蛋白质组 应用 

分 类 号：Q503[生物学—生物化学] Q789