大鼠脊髓损伤后坐骨神经电生理学以及损伤脊髓GAP-43蛋白表达的变化  

Changes of electrophysiology of the sciatic nerve and expression of GAP-43 protein in injured spinal cord tissue of rats

作　　者：谢晓[1] 高建新[1] 刘克敬[1] 禹卉[2] 杨贵忠[3] 

机构地区：[1]山东大学医学院生理学研究所,山东济南250012 [2]山东大学医学院形态教学实验室,山东济南250012 [3]山东大学医学院机能教学实验室,山东济南250012

出　　处：《山东大学学报（医学版）》2007年第5期474-477,共4页Journal of Shandong University:Health Sciences

基　　金：山东省科技攻关计划资助课题(2006GG3202044);山东省卫生厅科技计划项目(2004-2006)

摘　　要：目的:研究大鼠脊髓损伤后不同时间点坐骨神经兴奋阈值、动作电位(AP)幅度和传导速度,以及损伤脊髓GAP-43蛋白表达的改变。方法:采用改良的Allen′s重物坠落致大鼠脊髓损伤实验模型、电生理学和免疫组织化学染色。结果:大鼠SCI后1、3、7 d时,坐骨神经兴奋阈值升高,AP幅度和传导速度降低,呈时间依赖性改变;SCI后14 d,AP产生阈值、幅度和传导速度较SCI第7 d有所恢复。正常大鼠脊髓灰质神经元的胞浆和轴突中有少量GAP-43蛋白表达;SCI后第1、3、5、7 d时损伤脊髓周围区灰质GAP-43蛋白表达逐渐增强;在SCI后第14 d其阳性表达开始减弱。结论:大鼠SCI后能够导致坐骨神经电生理学兴奋性和传导性的降低,以及神经结构重构蛋白GAP-43表达增加。Objective： To investigate the changes of threshold, amplitude and conduction velocity of the action potential （AP） in the sciatic nerve and the expression of GAP-43 protein in rats with spinal cord injury （SCI）. Methods： Electrophysiological and immunohistochemistry techniques were used in experimental rats that were induced by the Allen＇s spinal cord weight-drop-injury method. Results： The increase of threshold, the decrease of amplitude and conduction velocity of AP in the sciatic nerve showed a time-dependent manner at 1 d, 3 d and 7 d following SCI. However, the above changes attenuated at 14 d following SCI. Expression of GAP-43 proteins progressively increased at 1 d, 3 d, and 7 d following SCI, and reached a peak at the 7th d. Conclusion： The excitability and conductibility of the sciatic nerve degrade and the expression of GAP-43 protein increases following SCI in rats .

关 键 词：脊髓损伤 坐骨神经 动作电位 GAP-43 大鼠 

分 类 号：Q42[生物学—神经生物学]