流动注射双安培法测定葛根素  

Flow Injection Biamperometric Analysis of Puerarin

作　　者：孙家娟[1] 尚永辉[1] 刘彬[1] 朱建勇[1] 

机构地区：[1]咸阳师范学院化学系,陕西咸阳712000

出　　处：《分析测试学报》2007年第2期225-227,231,共4页Journal of Instrumental Analysis

基　　金：陕西省教育厅科研计划项目(04JK303)

摘　　要：利用不可逆电对的双安培检测原理,建立了流动注射双安培直接检测葛根素的电化学新方法.使用经过恒电位预阳极化处理的双铂电极,通过耦合葛根素在一支电极上的氧化和氧化铂在另一支电极上的还原两个不可逆电极过程,构成流动注射双安培检测体系.在外加电位差为0.2 V时,pH 8.94的碳酸钠-碳酸氢钠缓冲溶液中,测得氧化电流与葛根素浓度在6.0×10^-6～1.0×10^-3 mol/L范围内呈线性关系（r=0.997 4,n=8）,检出限为1.0×10^-6 mol/L.连续40次测定6.0 ×10^-4 mol/L的葛根素,RSD=1.58%.用该方法对葛根素含量进行了测定,结果令人满意.A novel flow-injection electrochemical method for direct determination of puerarin was developed in this paper based on the biamperometric detection by an irreversible couple system. The applied voltage between two Pt electrodes was 0. 2 V, in which the electrocatalytic oxidation of puerarin occurred at one platinum electrode, coupled with the reduction of PtO at another Pt electrode. The linearity between current and puerarin concentration was obtained over the range of 6. 0×10^-6 - 1.0×10^-3 mol/L（r =0. 997 4, n = 8） with the detection limit of 1.0×10^-6 mol/L. The relative standard derivation of 1.58% was obtained for 40 successive determinations of 6. 0×10^-4 mol/L puerarin. The method has been successfully applied to the determination of puerarin in real samples with satisfactory results.

关 键 词：葛根素 流动注射分析 双安培法 不可逆电对 

分 类 号：O613.43[理学—无机化学]