毛细管电泳内标法测定糖果中胭脂红酸  

Determination of Carminic Acid in Sweet by Capillary Electrophoresis Using Internal Standard Method

作　　者：许元红[1] 唐亚军[1] 吴明嘉[1] 

机构地区：[1]中国科学院长春应用化学研究所国家电化学和光谱研究分析中心中国科学院研究生院,吉林长春130022

出　　处：《分析测试学报》2007年第1期136-138,共3页Journal of Instrumental Analysis

摘　　要：建立了胭脂红酸的毛细管电泳紫外测定方法。以未涂层融硅石英毛细管（55cm×75μm）为分离柱，3mmol／L硼砂（pH=8．92）为电泳介质，在分离电压为20kV，紫外检测波长为280nm的条件下对胭脂红酸进行测定。讨论了缓冲溶液、pH值、检测波长及分离电压的优化选择。以桂皮酸为内标对胭脂红酸进行定量测定，线性范围为3．92．100mg／L；检出限（S／N=3）为2mg／L。将该法应用于糖果中胭脂红酸含量的测定，方法简单可行且结果良好。A method of determining carminic acid by capillary electrophoresis with ultraviolet detection was developed. Carminic acid was detected under the following conditions： uncoated fused silica capillary （55 cm × 75 μm）, 3 mmol/L borax as background electrolyte （pH = 8.92 ）, 20 kV separation voltage and ultraviolet detection wavelength 280 nm. The selection of buffer, pH value, detection wavelength and separation voltage were discussed. Carminic acid was quantified with cinnamic acid as the internal standard. The calibration curve showed excellent linearity in the range of 3.92 - 100 mg/L. The limit of detection（S/N =3） was 2 mg/L. This method is feasible and simple and can be used to detect earminie acid in sweet.

关 键 词：毛细管电泳 胭脂红酸 糖果 

分 类 号：O657.8[理学—分析化学] TS264.4[理学—化学]