甘草抗病毒有效部位体外抗副流感病毒(Ⅲ型)作用的研究  

Activity against Parainfluenza Virus-3 of the Active Compound (GC3-1-4) from Radix Glycyrrhiza in vitro

作　　者：谢志平[1] 李洪源[1] 岳晓宏 王吉锡[1] 陈振华[1] 李鑫[1] 张建军[1] 朴英爱[3] 黄桂蓉[1] 陈洋[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院,黑龙江哈尔滨150086 [2]哈尔滨香坊区妇幼保健所,黑龙江哈尔滨150030 [3]黑龙江省药品检验所,黑龙江哈尔滨150001

出　　处：《中医药信息》2007年第3期37-39,共3页Information on Traditional Chinese Medicine

摘　　要：目的:研究甘草抗病毒有效部位(GC3-1-4)体外抑制副流感病毒(Ⅲ型)的作用。方法:采用煎煮法和柱层析法提取分离得到甘草抗病毒有效部位(CC3-1-4)。以病毒唑为阳性对照药,采用细胞病变抑制实验观察CC3-1-4在Hela细胞中对副流感病毒(Ⅲ型)的抑制作用。结果GC3-1-4半数申毒浓度(TQ50))为144．17μg／ml;抑制副流感病毒(Ⅲ型)的半数有效浓度(EC50)为12.82μg/ml,治疗指数(TI)为11．25;GC3-1-4对副流感病毒(Ⅲ型)的抑制作用存在量效反应关系;在感染后6h以內给药,GC3-1-4对副流感病毒(Ⅲ型)均有抑制作用。结论:GC3-1-4在Hela细胞中对副流感病毒(Ⅲ型)有抑制作用。Objective: To study the activity against Parainfluenza Virus - 3 of the Active Compound (GC3 - 1 - 4) from Radix Glycyrrhizae in vitro. Methods: The active component GC-3-1-4was extracted from Glycyrrhiza by hot water extraction, ethanol precipitation and column chromatography. The inhibitory effect was observed in Hela cells using Ribavirin as a positive control. Results: The median toxic concentration (TC50), median efficacious concentration (EC50) and treatment index (Tl)of GC3-1-4 were 144.17μg/ml,12.82μg/ml, 11.25, respectively. It showed inhibitory activity on PIV when GC3 - 1 - 4 was added Oh, 2h, 4h, 6h after infections. Conclusion: The study suggested that the GC - 3 - 1 - 4 has an obvious inhibitor to Parainfluenza Virus - 3 in Hela cell culture.

关 键 词：甘草 副流感病毒(Ⅲ型) 抗病毒作用 

分 类 号：R285.5[医药卫生—中药学]