寻常型银屑病患者治疗前后外周血中单个核细胞Foxp3 mRNA水平的检测分析  

The Investigation of Foxp3 mRNA in Peripheral Blood Mononuclear Cells of Patients with Psoriasis Vulgaris Before-and-after Treatment

作　　者：关宁[1] 沈柱[1] 刘玉峰[1] 

机构地区：[1]第四军医大学西京医院皮肤科,陕西西安710032

出　　处：《中国皮肤性病学杂志》2007年第5期257-259,共3页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金项目(No.30600558)

摘　　要：目的观察寻常型银屑病患者外周血单个核细胞中Fopx3 mRNA转录水平,并分析其治疗前后的变化特点,为探讨CD4+CD25+调节性T细胞(CD4+CD25+Treg)与寻常型银屑病免疫学发病机制的关系打下一定基础。方法采用巢式-聚合酶链式反应(Nested-PCR)的方法,检测25例寻常型银屑病患者和10例正常对照组的外周血单个核细胞中Foxp3 mRNA的转录水平,并对其中15例患者进行治疗前后的比较。以银屑病面积和严重度指数(psoriasis area andseverity index,PASI)评价疗效,并分析疗效与Foxp3 mRNA的转录水平的特点。结果25例寻常型银屑病患者外周血单个核细胞中Foxp3 mRNA水平(1.87±0.90)显著低于10例正常对照组(3.83±1.04)(P<0.05)。进一步分析显示:其中15例患者采用三联法治疗后,外周血单个核细胞中Foxp3mRNA转录水平显著升高,当PASI的评分为(3.2±2.97)时尤其明显(2.5 4±1.02)。结论寻常型银屑病患者外周血单个核细胞Foxp3 mRNA转录水平显著低于正常人,治疗后外周血单个核细胞中Foxp3mRNA水平显著升高,提示:CD4+CD25+Treg与寻常型银屑病免疫学发病机制密切相关。Objective To abserve the level of Foxp3 mRNA in peripheral blood of patients with psoriasis vulgafis,and analyze the change after the treatment . Methods The level of Foxp3 mRNA in the peripheral blood of the patients and healthy controls were detected by nested-RT-PCR. And there were 15 patients who compared with before-and-after treatment. The efficacy was evaluated by psoriasis area and severity index（PASI） , in order to analyze the relation between the efficacy and the level of foxp3 mRNA. Results Foxp3 mRNA expression was lower in the peripheral blood than that in the healthy controls （ 1.87 ± 0.90 VS 3. 83 ± 1.04 ,P 〈0.05）. After the therapeutic alliance,the level of Foxp3 mRNA was increased. The difference was obvious when the PASI was（3.2±2.97） and the the level of Foxp3 mRNA was（2.5 4±1.02） （P〈0. 05）, Conclusion The level of Foxp3 mRNA in the peripheral blood from patients was significantly lower than that in healthy controls, After the therapeutic alliance, the level of Foxp3 mRNA was increased with the PASI decreased.

关 键 词：寻常型银屑病 CD4^+CD25^+TREG FOXP3 NESTED-PCR 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]