黑素细胞与角质形成细胞混合培养模型的构建  

The Construction of Mixed Cultivating Model of Keratinocytes and Melanocytes in Vitro

作　　者：王正辉[1] 杨壮群[1] 屠军波[1] 张铁良[1] 李国光[1] 宋勇[1] 贺西京[2] 

机构地区：[1]西安交通大学医学院附属口腔医院口腔颌面整形外科,陕西西安710004 [2]西安交通大学医学院第二附属医院骨科,陕西西安710004

出　　处：《中国皮肤性病学杂志》2007年第5期283-285,共3页The Chinese Journal of Dermatovenereology

基　　金：2004年陕西省自然科学基金资助项目(2004C229)

摘　　要：目的体外构建黑素细胞与角质形成细胞直接接触的混合培养模型。方法以包皮组织作为细胞来源,分别培养角质形成细胞、黑素细胞;体外构建黑素细胞与角质形成细胞直接接触的混合培养模型。结果黑素细胞与角质形成细胞混合培养5天后出现浅灰色,继续培养,颜色逐渐加深,细胞生长良好。结论成功构建了黑素细胞与角质形成细胞直接接触的混合培养模型,为进一步研究色素问题奠定了基础。Objective To construct the mixed cultivating model of keratinocytes and melanocytes. Methods We used the foreskin tissue as a cell sourse, harvested and cultivated the melanocytes and keratinocytes respectively, and then constructed the mixed cultivating model of them. Results After cultivating for five days,the model showed a light gray color,and as the time went by, the color became thinner. Conclusion We have successfully constructed the mixed cultivating model of melanocytes and keratinocytes and provide requirements for further study.

关 键 词：混合培养模型 黑素细胞 角质形成细胞 

分 类 号：R322.99[医药卫生—人体解剖和组织胚胎学]