吴茱萸生物总碱的TLC-SERS研究  

TLC-SERS Study on Evodiamine in Evodia Rutaecarpa

作　　者：张进治[1] 汪瑗[2] 陈惠[2] 邵会波[2] 

机构地区：[1]北方工业大学理学院,北京100041 [2]首都师范大学化学系,北京100037

出　　处：《光谱学与光谱分析》2007年第5期944-947,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(29973026)资助

摘　　要：报道了将薄层色谱(TLC)与傅里叶变换表面增强拉曼散射(FT-SERS)联用,获得了中草药吴茱萸中六种生物碱分子光谱研究的新方法。薄层色谱将微克级吴茱萸生物总碱中吴茱萸碱(Evodiamine)、吴茱萸次碱(Rutaecarpine)、羟基吴茱萸碱(Hydroxyevodiamine)、吴茱萸酰胺(Evodiamide)、二氢吴茱萸次碱(Di-hydrorutaecarpine)和1,4-甲酰基二氢吴茱萸次碱(1,4-formyldihydrorutaecarpine)六种生物碱完全分离,应用薄层原位的傅里叶变换表面增强拉曼散射(TLC-FT-SERS)技术,获得吴茱萸生物碱分子的特征振动谱带,进而阐述了样品分子在银胶表面的吸附模式。五种吴茱萸生物碱分子以π电子与银晶体微粒相互作用,呈平面吸附;吴茱萸次碱以N原子的n电子与银晶体微粒作用。在薄层色谱原位,可明显观察到六种生物碱FT-SERS的光谱主要特征峰位。各生物碱分子拉曼散射获最大增强的是波数为1600cm-1表征苯环环伸缩振动的谱带;羟基吴茱萸碱谱图中可观察到波数为1342cm-1羟基变形振动谱带,明显区别于其他生物碱。采用此方法,不用标准品即获得六种生物碱的指纹光谱。研究结果表明了TLC-FT-SERS对中草药化学成分进行高灵敏度示踪指纹检测的可靠性和优越性。A new method for analyzing the ingredients of evodiamine（EV）, rutaecarpine（RU）, hydroxyevodiamine（HYD）, evodiamide（ED）, dihydrorutaecarpine（DRU） and 14-formyldihydrorutaecarpine（FDRU）in evodia rutaecarpa using high performance thin layer chromatography （TLC） and surface enhanced Raman spectroscopy （SERS） technique is reported. The character of this method is that standard samples are not needed. The results show that the characteristic spectral bands of EV, RU, HYD, and ED can be obtained from the TLC spot with microgramme of sample. The spectral band at 1 562 cm^-1 was obtained with great enhancement. Molecule absorbed in surface silver sol by π electrons in ring. The spectral bands of EV, RU, HYD and ED are obviously different due to their differences in structure. The TLC and SERS techniques standard samples are a convenient and speedy method to analyze chemical ingredients with high sensitivity for the study of the Chinese traditional medicine.

关 键 词：吴茱萸碱 吴茱萸次碱 薄层色谱 表面增强拉曼光谱 

分 类 号：O697.3[理学—化学]