己酮可可碱对充血性心力衰竭患者CD_4^+ Th细胞免疫调节的研究  

Effects of Pentoxifylline on immune modulation and mechanism of CD_4^+ Th cell in patients with congestive heart failure

作　　者：徐玉兰[1] 卢永昕[1] 万建平[1] 刘向阳[2] 荣书玲[1] 米少华[1] 高焱章[1] 

机构地区：[1]华中科技大学协和医院心内科华中科技大学同济医学院心血管病研究所,武汉430022 [2]广东省韶关市粤北医院心内科

出　　处：《临床内科杂志》2007年第5期301-303,共3页Journal of Clinical Internal Medicine

摘　　要：目的探讨己酮可可碱(PTX)对慢性充血性心力衰竭(心衰)患者外周血辅助性T淋巴细胞(Th)免疫调节的影响及其机制。方法采用流式细胞分析法,检测50例心衰患者和正常对照者(10名)外周血的CD4+Th1和Th2细胞,及其分泌的细胞因子干扰素-γ(IFN-γ)和白介素10(IL-10)在PTX干预下的变化。结果心衰对照组与正常对照组比较,分泌IFN-γ的Th1型细胞所占百分比明显增高而分泌IL-10的Th2型细胞所占百分比明显降低;PTX干预组与心衰对照组比较,分泌IFN-γ的Th1型细胞所占百分比明显降低而分泌IL-10的Th2型细胞所占百分比升高。结论心衰患者存在CD4+Th细胞亚群平衡偏移,而PTX可以逆转充血性心衰患者Th1/Th2细胞的失衡,起到免疫调节的作用,从而有可能使心衰患者从中获益。Objective To investigate the effects of PTX for the immune modulation and mechanism of CD^4＋ Th in patients with chronic heart failure. Methods peripheral blood cells were collected from 50 patients with CHF ,culturing in the presence pentoxifylline （4 ×10-4tool/L） and without pentoxifylline. Cytokine-producing Th lymphocytes were quantified by 3-color flow cytometry. Results Culturing with FIX the frequency of IFN-γ-producing Thl cells was significantly lower than in CHF group and the frequency of IL-10-producing Th2 cells was higher than in CHF group. Condusion The Thl/Th2 functional imbalance exits in patients with CHF. Pentoxifylline can reduce Thl development and increase Th2 cell-functions in CHF patients. Our findings suggest that Pentoxifylline can regulate the polarization of Thl/Th2 ,then it can regulate immune modulation ,this may be one of the mechanisms through which Pentoxifylline imlpreves heart function after CHF.

关 键 词：己酮可可碱 充血性心力衰竭 Th1淋巴细胞 Th1淋巴细胞 免疫调节 

分 类 号：R541.6[医药卫生—心血管疾病]