吸烟对肺功能和外周血T淋巴细胞IL-2mRNA的影响  

Effect of smoking on pulmonary function and the expression of IL-2mRNA in human peripheral blood T lymphocytes

作　　者：陈宏斌[1] 徐永健[1] 谢俊刚[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院呼吸内科,武汉430030

出　　处：《临床内科杂志》2007年第5期304-306,共3页Journal of Clinical Internal Medicine

基　　金：国家自然科学基金资助项目(30400194和30570794)

摘　　要：目的探讨吸烟对健康人肺功能和外周血T细胞IL-2mRNA表达的影响。方法随机选择健康吸烟者和非吸烟者各24名,检测肺功能指标和外周血T淋巴细胞中IL-2mRNA表达。结果吸烟组中FEV1、FEV1/FVC%、MMEF75/25较对照组有明显下降(P<0.05);吸烟组中IL-2mRNA较对照组有显著性增高(P<0.05)。肺功能中FEV1/FVC%随IL-2mRNA的表达水平增加呈降低趋势(r=-0.758,P<0.05)。结论吸烟者在尚无临床症状时,肺功能已经受损。吸烟可能通过增加T淋巴细胞IL-2mRNA的表达促进炎症反应导致肺功能损害。Objective To explore the effect of smoking on pulmonary function and IL-2mRNA in human peripheral blood T lymphoeytes. Methods We randomly selected 24 healthy smokers as smoking group and 24 healthy nonsmokers as control group. To detected their pulmonary function by portable spirometers and the expression of the IL-2mRNA in T lymphoeytes were determined by reverse transcription polymerase chain reaction （RT-PCR）. Results The FEV1、FEV1/ FVC % and MMEF75/25 in smoking group had significantly decreased compared with control group（ n =24 ,P 〈0.05） ;the expression of the IL- 2mRNA in T lymphoeytes from smoking group had significantly increased compared with control group （ n = 24 ,P 〈0.05）. The pulmonary function had strongly negative correlation with the expression level of IL- 2mRNA（r=-0.785,n =24,P 〈0.05）..Conclusion Although smokers did not show clinical symptoms,their pulmonary function had been reduced;It is suggested that smoking may increased lung inflammatory response by inducing the expression level of IL-2mRNA.

关 键 词：吸烟 肺功能 T淋巴细胞 IL-2MRNA 

分 类 号：R563.9[医药卫生—呼吸系统]