高效液相色谱法测定蔬菜中精喹禾灵残留研究  

Determination of quizalofop-p-ethyl residue in vegetables by HPLC

作　　者：马建明[1] 赵立达[1] 马旭亮[1] 

机构地区：[1]浙江省慈溪市疾病预防控制中心,浙江慈溪315300

出　　处：《中国卫生检验杂志》2007年第5期827-827,924,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立快速、简便、灵敏的蔬菜中精喹禾灵残留的高效液相色谱检测方法。方法:蔬菜样品经甲醇/丙酮/盐酸溶液提取,通过中性氧化铝和弗罗里硅土的混合柱净化,在XDB C8(150 mm×4.6 mmi.d.,5μm)色谱柱上,以甲醇/乙酸-乙酸铵溶液(10 mmol/L,pH 6.2,80/20,v/v)为流动相,流速为0.8 ml/min,柱温为35℃,紫外检测波长为235 nm。结果:蔬菜中精喹禾灵的平均回收率在83.2%-94.2%范围,RSD小于5.5%,线性范围为0.5-50.0 mg/L(r=0.9992)。结论:建立的方法用于蔬菜中精喹禾灵残留的测定,具有操作简便、快速、准确的优点。Objective:To establish a simple,accurate and sensitive method for the determination of quizalofop-p-ethyl in vegetables by liquid chromatography with ultraviolet detection.Methods:Samples were extracted by methanol/acetone/2.0 mol/L hydrochloric acid and cleaned up on a neutral alumina and florisil mixed column.A satisfactory separation was achieved on a zorbax XDB C8 column(150 mm×4.6 mm i.d.,5 μm)using methanol/acetic acid-ammonium acetate(10 mmol/L,pH 6.2,80/20,v/v) as the mobile phase at a constant temperature of 35℃ at a flow rate of 0.8 ml/min.Detection was performed on an ultraviolet detector at 235 nm.Results:The linear range was 0.5~50.0 mg/L and the limit of quantification was found to be 0.5 mg/L.The extraction recoveries were between 83.2%~94.2%,the RSD was less than 5.5%.Conclusion:This described method is found to be simple,rapid and accurate for the determination of quizalofop-p-ethyl in vegetables.

关 键 词：高效液相色谱 蔬菜 精喹禾灵 

分 类 号：O657.72[理学—分析化学]