periostin在过度增生性瘢痕的表达及其与TGF-β1和受体的相关性  

The expression of periostin in hyperplasic scars and the relations to TGF-β1 and its receptors

作　　者：王齐[1] 聂芳菲[1] 赵霞[1] 秦泽莲[1] 

机构地区：[1]北京大学第三医院成形外科,北京100083

出　　处：《中华整形外科杂志》2007年第3期229-232,共4页Chinese Journal of Plastic Surgery

基　　金：国家自然科学基金（#30471793）;中国教育部“211”经费资助课题

摘　　要：目的检测periostin在过度增生性瘢痕中的表达，研究其与TGF-β1和受体的相关性，探讨periostin与瘢痕过度增生的关系。方法采用RT-PCR法检测瘢痕疙瘩、增生性瘢痕、正常皮肤组织中periostin、TGF-β1，及其受体Ⅰ、Ⅱ的mRNA表达，Western blot法检测periostin的蛋白表达。结果在基因水平，periostin在瘢痕疙瘩的表达高于正常皮肤，TGF-β1在瘢痕疙瘩的表达高于增生性瘢痕和正常皮肤，TGF-βRⅠ在瘢痕疙瘩的表达高于增生性瘢痕和正常皮肤，上述差异有统计学意义（P〈0．01）；TGF-βRⅡ的表达在3组间差异无统计学意义。在蛋白水平，periostin在瘢痕疙瘩和增生性瘢痕的表达均高于正常皮肤（P〈0．05）。periostin和TGF-β1的表达呈正相关（P〈0．01）。结论periostin在瘢痕疙瘩组织中表达增高，是瘢痕相关基因；其在瘢痕疙瘩形成中可能发挥重要作用，该作用与TGF-β1密切相关。Objective To probe into periostin＇ s role in the pathological mechanism of hyperplasic scars, by examining the expression of periostin in hyperplasic scar tissues. To investigate the correlations between periostin and TGF-β1 , TGF-β R Ⅰ , TGF-β R Ⅱ . Methods RT-PCR was used to assess the mRNA expression levels of TGF-β1, TGF-β R Ⅰ, TGF-β R Ⅱ in three kinds of tissues, which are keloid（ K）, hypertrophic scar（HS） and normal skin（SK）. The preotein expression of periostin was measured with Western blotting. Results The mRNA level of periostin in K was higher than that in SK. The mRNA expression of TGF-β1 in K was higher than that in HS and SK. The mRNA level of TGF-β RⅠ in K was higher than that in HS and SK. The significances above all was at P 〈 0.01. The protein expression level of periostin in HS increased, compared with that in SK （P 〈 0.05）. Periostin was related to TGF-β1 positively（ P 〈 0.01）. Conclusions The periostin＇ s expression is increased in keloids. Periostin is a cicatrix specific gene. Periostin appears to play an important role in the formation of keloids, which is related to TGF-β1 closely.

关 键 词：PERIOSTIN TGF-Β1 受体 瘢痕疙瘩 增生性瘢痕 

分 类 号：R686[医药卫生—骨科学]