系列可吸收珊瑚羟基磷灰石的制备及作为骨组织工程支架材料的实验研究  

The preparation of series of the controllable degradation coral-hydroxyapatite（SCHA-200R） and the experiment studies on its application as the scaffold in bone tissue-engineering

作　　者：高瞻[1] 毛天球[1] 陈富林[1] 何黎升[1] 侯锐[1] 杨耀武[1] 吴小箐[2] 

机构地区：[1]第四军医大学口腔医学院口腔颌面外科,西安710032 [2]西安交通大学精细功能电子材料与器件国家专业实验室

出　　处：《中华整形外科杂志》2007年第3期236-240,共5页Chinese Journal of Plastic Surgery

基　　金：国家973资助项目（G1999054308）;国家自然科学基金（30270373）项目

摘　　要：目的研制性能稳定的系列可吸收珊瑚羟基磷灰石。方法①珊瑚在高温高压条件下与磷酸氢二铵反应，反应后分析三组转化材料的物相组成和晶体构型。②观察反应产物生物降解情况。进行金属元素分析，了解毒性元素的含量，并对其进行溶血实验、细胞毒性实验和骨相容性实验。结果①在不同条件下进行水热反应，生成不同碳酸钙，羟基磷灰石比例的梯度材料。②此梯度材料保持了多孔网状结构的特点，其小梁超微结构可见表面有一薄层致密的物质。③转化材料生物相容性良好。结论①在不同反应条件下，可形成梯度珊瑚羟基磷灰石材料。②转化材料保持了多孔网状结构，表面有一薄层羟基磷灰石。③转化材料可作为骨组织工程的支架材料。Objective Fabricate series of the controllable degradation coral-hydroxyapatite. Methods The natural coral undergo a chemical reaction with （ NH4 ）2 HPO4 at high temperature and pressure for different time-lengths. After getting the products, the components and the spacial structures were analyzed. Observe the biologic degradation of the reaction products and analyze the metal elements and their contents. Heamolysis tests, eytotoxity tests and bone compatibility tests were performed to assess the biocompatibility of the products. Results When hydrothermal reactions happened under different conditions, the different gradients of CaCO3/hydroxyapatite materials were produced. These types of materials kept the characteristic of interconnected micro-porous network structures. A thin layer of compact material can be seen on the surface of its trabecula uhra-micro structure. The SCHA-200R has a good biocompatibility. Conclusions Gradient HA （SCHA-200R） materials can be formed by adjusting the same temperature, same pressure and different time-length of the reaction. This kind of gradient material keeps the quality of micro-porous network structures. The SCHA-200R is a potential candidate scaffold for bone tissue engineering.

关 键 词：组织工程 支架材料 珊瑚 羟基磷灰石 骨髓间充质成骨细胞 

分 类 号：R686[医药卫生—骨科学]