脂肪瓣延迟术后瓣内脂肪细胞移植增加成活的实验研究  

Experimental study of increasing effect of the fat flap tissue after delay operation on its free graft survival volume and duration

作　　者：钟晓春[1] 倪有娣[1] 何晓升[1] 戚富琴[1] 杨天鹏[1] 

机构地区：[1]杭州市第二人民医院,杭州 310015

出　　处：《中华整形外科杂志》2007年第3期251-253,共3页Chinese Journal of Plastic Surgery

基　　金：杭州市卫生局科研基金（2004BZ206）,杭州师范学院科研启动基金（50427010604）

摘　　要：目的探讨脂肪瓣延迟术后瓣内脂肪细胞移植成活率和成活时间。方法以兔为模型，在腹股沟处形成脂肪瓣。12h时，用Elisa法检测瓣内血管内皮生长因子（VEGF）的表达，21d后把瓣内脂肪剪成颗粒状移植至兔的背部，移植后1、3、6、9和12个月取出移植脂肪组织，进行肉眼观察，称重和组织切片检查，用CD34染色检测血管增生情况。结果在脂肪瓣形成12h后VEGF显著增高（P〈0．05），移植后1和3个月，实验组移植脂肪血管数显著高于对照组（P〈0．01），成活率未见差异（P〉0．05），但存活时间明显延长，瓣内脂肪细胞在移植后12个月仍然存活，而对照组6个月时已基本吸收。结论脂肪瓣延迟术后移植瓣内脂肪细胞能增加成活率及成活时间，可为临床脂肪移植提供新的思路和方法。Objective To observe the effects of the fat flap tissues after delay operation on free fat-graft survival rate and duration. Methods The delay operation of fat flaps was performed in the inguinal region of a rabbit. Expression of VEGF was assayed using Elisa method after 12 hours of flap delay. The fat flaps were harvested and cut into pieces after 21 days. A subdermal pocket was created in each side of the dorsal midline of a rabbit, the fat pieces were grafted randomly into a pocket and the normal fat pieces into the other pocket as control. After 1, 3, 6, 9 and 12 months of implantation, the grafted fats were harvested, gross observation, weight measurement and histology were carried out. Number of the vessels stained with anti-CD34 antibody was counted out. Results VEGF concentrations in flaps were significantly higher （ P 〈 0.05 ）. The density of vessels in experimental groups increased significantly compared with that in control groups at 1 and 3 months, respectively （P 〈0.01）, and no significant differences in the survival rate of fat tissues between experimental and control groups were observed at 1 and 3 months （P 〉 0.05）. The fat cells from the flaps survived after 12 months of fat plantation, while those in control groups disappeared after 6 months. Conclusions The survival rate and duration of grafted fat could be increased implanting the fat tissues from delayed fat flap, which may provide researchers with a new method for fat graft.

关 键 词：脂肪瓣 延迟术 脂肪移植 成活 

分 类 号：R686[医药卫生—骨科学]