胶束毛细管电泳测定枇杷叶中熊果酸和齐墩果酸的含量  

MECC determination of ursolic acid and oleanolic acid in leaf of Eriobotrya japonica

作　　者：肖美添[1,2] 叶静[2] 黄雅燕[2] 刘青[2] 

机构地区：[1]华侨大学制药工程研究所 [2]华侨大学化工与制药工程系,泉州362021

出　　处：《药物分析杂志》2007年第5期724-727,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立测定枇杷叶中熊果酸和齐墩果酸含量的毛细管电泳方法。方法:采用胶束毛细管电泳法(MECC),未涂层弹性石英毛细管(75 μm×60 cm,有效长度50 cm);压力进样,压力:3.0×10~3 Pa;进样时间:5 s;分离电压:24 kV;柱温:25℃;检测波长:200 nm;运行缓冲液:30 mmol·L^(-1) SDS,20 mmol·L^(-1)羟丙基-β-环糊精,5%乙腈,40 mmol·L^(-1)硼砂溶液(pH9.4);运行时间:30 min。结果:测得熊果酸和齐墩果酸的线性范围分别为42.5～680 μg·mL^(-1)(r=0.9989)和33.0～660μg·mL^(-1)(r=0.9994),平均回收率(n=5)分别为98.1%(RSD 为3.3%)和97.8%(RSD 为2.9%)。结论:方法准确可靠,适合于枇杷叶中熊果酸和齐墩果酸的含量测定。Objective： To establish an HPCE method for the determination of ursolic acid and oleanolic acid in leaf of Eriobotrya japonica. Method：The separation was performed on a fused and uncoated silic capillary of 75 μm×60 cm （50 cm of effective length）. 40 mmol·L^-1 borax （ pH 9. 4 ） containing 30 mmol·L^-1 sodium dodecyl sulfate （SDS） ,20 mmol·L^-1 hydroxypropyl -β - cyclodetrin （ HP -β- CD） and 5% acetonitrile was used as running buffer. Samples were injected hydrodynamically（5 s ,3.0 ×10^3 Pa）into the capillary. The applied voltage was 24 kV and the wavelength of detection was set at 200 nm. The temperature was kept at 25℃. The running time was 30 min. Results： The calibration curves were linear in the range of 42. 5 - 680 μg·mL^-1 （ r = 0. 9989 ） for ursolic acid and 33.0 - 660 ·mL^-1（ r = 0. 9994 ） for oleanolic acid. The average recoveries（ n = 5 ） were respectively 97. 1% and 97.6% （RSD 3.3% and 2. 9% ）for ursolic acid and oleanolic acid. Conclusion：This method is accurate and reliable, and suitable for the determination ursolic acid and oleanolic acid in leaf of Eriobotrya japonica.

关 键 词：枇杷叶 熊果酸 齐墩果酸 胶束毛细管电泳 

分 类 号：R917[医药卫生—药物分析学]