家兔扩张囊胚玻璃化冷冻保存技术的研究  

Studies on Cryopreservation of Rabbit Expanded Blastocysts by Vitrification

作　　者：朱士恩[1] 曾申明[1] 张忠诚[1] 

机构地区：[1]中国农业大学动物科技学院

出　　处：《中国养兔杂志》1997年第2期16-20,共5页Chinese Journal of Rabbit Farming(CJRF)

摘　　要：本试验对家兔扩张囊胚的玻璃化冷冻保存技术进行了探讨。首先在２０℃室温下，将扩张囊胚直接移入ＥＦＳ４０溶液中短时间平衡后，直接投入液氮中冷冻保存（一步法）；或胚胎在１０％～２０％ＥＧ（或ＥＦＳ２０）溶液中经预先处理后，再移入ＥＦＳ４０中冷冻保存（二步法），解冻后的胚胎发育率达到９５％～１００％。当室温提高到２５℃时，一步法和二步法冷冻的胚胎均得到了较高的发育率（８９％～９０％）。利用２０℃条件下一步法即２分钟平衡后冷冻的胚胎移植后，妊娠受体的产仔率为２９．２％（７／２４），同对照组产仔率３２．４％（１１／３４）相比无显著性差异（Ｐ＞０．０５）。Cryopreservation of expanded blastocysts by vitrification was studied The rabbit expanded blastocysts were equilibrated in EFS40 for short time at 20℃ and directly plunged into liquid N 2 (one-step method) or the embryos were pretreated in 10%～20% EG(or EFS20) at 20℃ and cryopreserved in EFS40(two-step method) The rates of development were 95%～100% after the frozen embryos were thawed When the experiments were carried out at 25℃,the developmental rates of thawed embryos were 89%～90% in both one-step and two-step method The embryo cryopreserved by one-step method were transferred into recipients The rates of embryos developing into young pups were 29 2%(7/24) and the rates were 32 4%(11/34) in the control There were no significant difference between the experiment and the control(P>0 05)

关 键 词：家兔 扩张囊胚 玻璃化 冷冻 移植 

分 类 号：S829.1[农业科学—畜牧学]