玉米自交系RAPD分析中的样品选择  

Sampling in RAPD Analysis of Corn Inbreds

作　　者：李连城[1] 傅骏骅[1] 刘新芝[1] 彭泽斌[1] 黄长玲 

机构地区：[1]中国农业科学院作物育种栽培研究所,北京100081

出　　处：《玉米科学》1997年第1期6-9,共4页Journal of Maize Sciences

摘　　要：近年来分子标记技术在我国农业科研中的应用已受到了广泛的重视。分子标记样品的代表性如何将对研究结果产生影响，因此，研究分子标记的取样问题具有一定的现实意义。本文对以玉米自交系为样品，进行分子标记分析时的取样技术进行了探讨。在对玉米自交系中黄204基因组DNA进行RAPD分析时，同一引物8个单株及其混合样品的扩增结果中，单株6在564bp处比其它单株多一条较强的谱带，而8个单株的混合样品的扩增结果中也有一条同样的谱带。而丹340、旅9、5003等其它自交系的10个单株及其混合样品的RAPD谱带是一致的。说明中黄204单株6的遗传背景与其它单株不同，不能代表中黄204，如用它们的混合样品进行RAPD分析，就会得出错误的结果。可以看出，分子标记分析中的取样技术是检测结果可靠性不可忽视的重要因素之一。Molecular marker technology has ben applied extensively in agricultural sciences recent-ly. It is important for sampling in molecular marker investigation because the sampling directly affect themolecular level result, The sampling technology of molecular marker analysis was studied for maize inbredin this repot. We analysed the RAPO bands of each of eight or ten plants genomic DNA and their mixedDNA sample for each of inbred Zhong huang 204, Dan340, us and 5003 with the same primer. In our ex-periment, the amplification products of every plant genomic DNA of each inbred except Zhonghuang 204 isthe same as that products of their ndxed sample. There was a special main amplified band which foratedin 564 hp of the sixth of eight plants gendrie DNA of Zhonghuang 204, that my band is also exsistentin their whxed sample, but is not exsistent in the other plants. fo the sixth plant DNA sample can, t standfor inbred Zhonghuang 2O4, the RAP result will be wnng if eded Plans DNA sample was used as tem-plete to amplify. Hance, saxnpling is very important in molecular eder experiment. In general, forve re-sult indicated that unstandaul inbtal genolnic DNA samle must be discaldd in rnolecular level indicatedthat unstandaal inbred genomic DNA same must be discwhed in meecular level analysis.

关 键 词：玉米 自交系 取样 

分 类 号：S513.032[农业科学—作物学]