脑心通胶囊对体外模拟脑缺血再灌注损伤大鼠脑微血管内皮细胞的保护作用  

Protective Effect of Naoxintong Capsules on Cultivated Rat Cerebral Microvascular Endothelial Cells Subjected to Simulated Cerebral Ischemia and Reperfusion in Vitro

作　　者：刘振权[1] 徐秋萍[2] 张文生[1] 郭建友[3] 杜树山[1] 王树强[1] 陈超杰[1] 顾彬[1] 

机构地区：[1]北京师范大学资源学院资源药物与中药资源研究所教育部资源药物工程研究中心 [2]北京中医药大学中药学院中药药理系,北京100102 [3]中国科学院心理研究所,北京100101

出　　处：《中国药学杂志》2007年第10期733-736,共4页Chinese Pharmaceutical Journal

基　　金：北京市教委新医药学科群项目(227105803)

摘　　要：目的观察脑心通胶囊对体外模拟脑缺血再灌注损伤大鼠脑微血管内皮细胞的保护作用。方法建立体外培养大鼠脑微血管内皮细胞模拟脑缺血再灌注损伤模型,模拟脑缺血3h,再灌注1,3,6,12,24,36,48,72h后损伤内皮细胞的活性、死亡率变化以及脑心通胶囊的影响,测定体外培养大鼠脑微血管内皮细胞模拟脑缺血3h,再灌注24h时脑心通胶囊含药血清对细胞裂解液SOD活性、MDA及NO含量的影响。结果体外培养的大鼠脑微血管内皮细胞在模拟缺血再灌注损伤后,细胞内线粒体活力下降,死亡率升高,脑心通胶囊0.24,0.48g.kg-1能不同程度改善细胞损伤(P<0.05,P<0.01),明显提高裂解液中SOD活性(P<0.05,P<0.01),降低裂解液中MDA和NO含量(P<0.05,P<0.01),对大鼠脑微血管内皮细胞模拟脑缺血再灌注损伤有明显的保护作用。结论脑心通胶囊对体外培养大鼠脑微血管内皮细胞模拟脑缺血损伤有保护作用,其机制可能与抗脂质过氧化作用有关。OBJECTIVE To observe the protective effects of Naoxintong capsule （NXTJN） on the cultivated rat cerebral microvascular endothelial cells （rCMEC） subjected to simulated cerebral ischemia and reperfusion in vitro. METHODS rCMEC activity, motatity rate and the effects of NXTJN were observed after simulated cerebral ischemia for 3 h and reperfusion for 1,3,6,12,24,36,48 and 72 h, respectively. The change of SOD activity, MDA content and NO content of cell lysate and the effects of NXTJN were observed ~ after simulated cerebral ischemia of 3 h and reperfusion for 24 h. RESULTS The rCMEC viability was decreased and the motality rate was increased significantly in various ischemia reperfusion durations and NXTJN could affect the changes in these criteria. NXTJN at the dose of 0. 24 and 0. 48 g · kg^-l increased the activity of SOD and reduced the content of MDA and NO in lysate of rCMEC subjected to simulated cerebral ischemia for 3 h and reperfusion for 24 h （ P 〈 0. 05, P 〈 0. 01 ）. CONCLUSION NXTJN protects cerebral ischemia reperfusion injury in rCMEC in vitro. And the mechanism is probably related to the suppression of the generation of NO and reduction of lipid peroxide action.

关 键 词：恼心通胶囊 脑缺血再灌注损伤 脑微血管内皮细胞 超氧化物歧化酶 丙二醛 一氧化氮 

分 类 号：R965[医药卫生—药理学]