重组腺病毒载体介导人DeltaNp73α转染对树突状细胞凋亡的抑制作用  

Inhibitory effect of recombinant adenovirus vector-mediated transfection of DeltaNp73α on dendritic cell apoptosis

作　　者：胡义杰[1] 范士志[1] 蒋耀光[1] 李志平[1] 陈建明[1] 何勇[1] 

机构地区：[1]第三军医大学附属大坪医院野战外科研究所胸心外科,重庆400042

出　　处：《第四军医大学学报》2007年第10期877-880,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30600611);第三军医大学校中青年基金项目(XG200540)

摘　　要：目的研究人DeltaNp73α基因重组腺病毒转染人脐血来源树突状细胞(DC)对其成熟状态及其凋亡水平的影响.方法用Adeasy系统构建人DeltaNp73α基因重组腺病毒;并通过增强离心法将其转染至人脐血来源DC;流式细胞仪分析转染后DC的成熟状态以及DC的凋亡水平.结果成功构建了人DeltaNp73α基因重组腺病毒并高效转染DC;人DeltaNp73α基因重组腺病毒转染提高了DCCD83,HLA-DR的表达,并能显著抑制其凋亡水平.结论人DeltaNp73α基因重组腺病毒转染DC,促进DC的成熟并抑制其凋亡,将有效地提高DC疫苗的抗原呈递能力.AIM： To investigated the effect of transfection with DeltaNp73α recombinant adenovirus on the maturation and vitality of dendritic cells from human umbihcal blood. METHODS： DeltaNp73α recombinant adenovirus were constructed with Adeasy system. Immature dendritic cells were isolated from human umbilical cord blood, and cultured in the presence of interleukin-4 and granulocyte/macrophage colony-stimulating factor, and then transfected with the adenovirus vector with centrifugal force method. Viability and maturation state of dendritic cells after transfection were assessed by flow cytometric analysis. RESULTS： DeltaNp73α recombinant adenovirus vector was constructed successfully; and DeltaNp73α expression in the transfected dendritic cells was confirmed by RT-PCR and Western Blot. The expressions of CD83, HLA-DR and the vitality of transfected dendritic cells were increased. CONCLUSION： Transfection with DeltaNp73α recombinant adenovirus promotes the maturation and inhibits the apoptosis of dendritic cells, which enhances effectively the antigen presentation of dendritic cell vaccine.

关 键 词：DeltaNp73α 重组 遗传 腺病毒科 树突细胞 树突细胞疫苗 

分 类 号：R392.12[医药卫生—免疫学]