Study the effects of divalent metallic ions on the combination of DNA and histones with fluorescence anisotropy assays  

作　　者：LIU YuYing WANG PengYe DOU ShuoXing XIE Ping XI XuGuang 

机构地区：[1]College of Science, China Agricultural University, Beijing 100083, China [2]Laboratory of Soft Matter Physics, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100080, China [3]Laboratoire de Biotechnologies et Pharmacologie Genetique Appliquee CNRS UMR 8113, Ecole Normale Superieure de Cachan, 61 Avenue du Pre'sident Wilson, 94235 Cachan cedex, France

出　　处：《Chinese Science Bulletin》2007年第9期1166-1171,共6页

基　　金：Supported by the National Natural Science Foundation of China (Grant Nos.60025516 and 10334100), the Innovation Project of the Chinese Academy of Sci-ences, and the CNRS (to Xi Xuguang)

摘　　要：The effects of divalent ions(Mn2+,Mg2+,Ca2+) on the interaction between DNA and histone are studied using a fluorescence anisotropy assay. Fluorescence anisotropies of DNA and DNA-histone in the presence of divalent ions(Mn2+,Mg2+,Ca2+) are measured. The results indicate that histone reduces the fluorescence anisotropy of lambda DNA while the divalent ions(Mn2+,Mg2+,Ca2+) significantly enhance the fluorescence anisotropy. Compared to the case of DNA incubated with histone alone,there are more histones binding to DNA when divalent ion,histone and DNA are incubated together. We also find that Mn2+ makes the DNA-histone complexes more condensed than the other ions do.The effects of divalent ions （Mn^2＋, Mg^2＋, Ca^2＋） on the interaction between DNA and histone are studied using a fluorescence anisotropy assay. Fluorescence anisotropies of DNA and DNA-histone in the presence of divalent ions （Mn^2＋, Mgv, Ca^2＋） are measured. The results indicate that histone reduces the fluorescence anisotropy of lambda DNA while the divalent ions （Mn^2＋, Mg^2＋, Ca^2＋） significantly enhance the fluorescence anisotropy. Compared to the case of DNA incubated with histone alone, there are more histones binding to DNA when divalent ion, histone and DNA are incubated together. We also find that Mn^2＋ makes the DNA-histone complexes more condensed than the other ions do.

关 键 词：二价金属离子 DNA 组蛋白 荧光各向异性度检验 

分 类 号：Q591.2[生物学—生物化学] Q523