Structural analysis of the promoter of tomato 1-aminocyclopropane-1-carboxylate synthase 6 gene(Le-ACS6)  

作　　者：LIN JingYu FAN Rong WAN XiaoRong CHARNG Yee-yung WANG NingNing 

机构地区：[1]Department of Plant Biology and Ecology, Nankai University, Tianjin 300071, China [2]Institute of BioAgricutural Sciences, Academia Sinica, Nankang, Taipei 11529, China

出　　处：《Chinese Science Bulletin》2007年第9期1217-1222,共6页

基　　金：Supported by the National Natural Science Foundation of China (Grant Nos. 30470174 and 30070075) ;Natural Science Foundation of Tianjin City (Grant No. 05YFJZJC00800)

摘　　要：Ethylene plays an important role in the regulation of many growth and developmental processes of higher plants. In tomato,Le-ACS6,a member of the ACC synthase multigene family involved in system 1 ethylene biosynthesis during fruit ripening,is subject to negative feedback regulation by ethylene. To identify the cis-elements that are responsible for the negative feedback control,we established an in vitro transient assay system employing particle bombardment on mature-green tomato fruit pericarp to examine the expression of a luciferase(LUC) reporter gene driven by a 5′-serially deleted Le-ACS6 promoter. The results localized putative cis-elements required for negative ethylene-response between -347 and -266 upstream from the translational start site ATG. Several lines of stable transformation of the Le-ACS6 promoter and GUS reporter fusion gene containing internal deletion from -347 to -266 were generated. The expression pattern of the GUS reporter showed that removal of the nucleotides from -347 to -266 completely eliminated the response of the Le-ACS6 promoter to exogenous ethylene.Ethylene plays an important role in the regulation of many growth and developmental processes of higher plants. In tomato, Le-ACS6, a member of the ACC synthase multigene family involved in system 1 ethylene biosynthesis during fruit ripening, is subject to negative feedback regulation by ethylene. To identify the cis-elements that are responsible for the negative feedback control, we established an in vitro transient assay system employing particle bombardment on mature-green tomato fruit pericarp to examine the expression of a luciferase （LUC） reporter gene driven by a 5＇-serially deleted Le-ACS6 promoter. The results localized putative cis-elements required for negative ethylene-response between -347 and -266 upstream from the translational start site ATG Several lines of stable transformation of the Le-ACS6 promoter and GUS reporter fusion gene containing internal deletion from -347 to -266 were generated. The expression pattern of the GUS reporter showed that removal of the nucleotides from -347 to -266 completely eliminated the response of the Le-ACS6 promoter to exogenous ethylene.

关 键 词：番茄 乙烯 1-氨基环丙烷-1-羧酸盐合酶6基因 Le-ACS6 启动子 结构分析 

分 类 号：S641.2[农业科学—蔬菜学] Q946.885.7[农业科学—园艺学]