人骨髓MSC对PHA活化T细胞周期以及表型和细胞因子分泌的影响  

Effects of MSC on cell cycle, phenotype and cytokine secretion of T cells activated by PHA

作　　者：栾希英[1,2] 张光波[1] 胡玉敏[1] 於葛华[1] 王明元[1,3] 段巧艳[1] 段祥[1] 张学光[1] 

机构地区：[1]苏州大学医学生物技术研究所江苏省干细胞重点实验室 [2]滨州医学院免疫学教研室,山东滨州256603 [3]苏州市红十字中心血站,江苏苏州215007

出　　处：《细胞与分子免疫学杂志》2007年第5期402-405,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国防预研项目基金资助(A3820060130);江苏省干细胞研究重点实验室基金资助(2005年)

摘　　要：目的研究人骨髓间充质干细胞(MSC)对T细胞周期和活化的影响,探讨MSC对T细胞增殖抑制的作用机制。方法Ficoll密度梯度离心法分离纯化人骨髓MSC,体外扩增培养3代后用于实验。应用流式细胞术分析MSC对PHA作用下T细胞周期和早期表型CD25、CD69表达的影响,ELISA法检测细胞因子水平。结果人骨髓MSC体外可使PHA刺激下的T细胞滞留于细胞周期的G0/G1期,下调活化T细胞早期表型CD25、CD69的表达,抑制IL-2、IFN-r的分泌。结论人骨髓MSC体外可通过对T细胞周期的影响抑制其活化和增殖。AIM： To study the effect of human bone marrow derived mesenchymal stem cell （ MSC ） on T cell cycle and activation, and to investigate the inhibitory effect of MSC on T cell proliferation and the underlying mechanism. METHODS： Human bone marrow derived MSC were isolated by gradient centrifugation, then in vitro MSC were cultured, expanded, and were used in test after third passage. FCM analysis and ELISA were used to investigate the effects of MSC on the early activation marker expression of T cells, cell cycle and cytokine secretion. RESULTS： T cells stimulated by PHA in the presence of MSC were arrested at G0/G1 phase. The expression of the early activation marker CD25 and CD69 of T cells was inhibited in the presence of MSC both in CD4^＋ and CD8^＋ T cell subpopulation. MSC caused a sharp decrease of cytokine secretion in IL-2 and IFN-γ. CONCLUSION： Human bone marrow derived MSC can suppress the activation and proliferation of T cells by altering T cell cycle.

关 键 词：骨髓间充质干细胞 T细胞 免疫调节 细胞周期 

分 类 号：R392.4[医药卫生—免疫学]