脂肪基质干细胞的分离培养及其作为软骨种子细胞的研究  

Isolation, cultivation of adipose-derived mesenchymal stem cells and its chondrogenic ability

作　　者：马钰[1] 李青[1] 赵大庆[1] 王淑芳[1] 李望舟[2] 闵婕[1] 谷雨[1] 

机构地区：[1]第四军医大学西京医院病理科,陕西西安710032 [2]第四军医大学唐都医院烧伤整形科,陕西西安710038

出　　处：《细胞与分子免疫学杂志》2007年第5期463-465,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：全军医药卫生科研基金课题资助(06MA233)

摘　　要：目的研究人体脂肪基质干细胞(ADSC)分离培养的方法,探讨其在体外向成软骨细胞诱导分化的能力。方法取临床吸脂所得脂肪组织,胶原酶消化分离后,接种于培养基中进行原代培养,传至一代时换软骨诱导培养液进行成软骨诱导分化,定期通过阿尔辛蓝染色、天狼猩红染色及免疫组化等方法进行证实。结果ADSC在体外成软骨诱导后,可向软骨细胞分化,经诱导的细胞可分泌软骨细胞特异性的Ⅱ型胶原以及硫酸蛋白多糖。结论在适当诱导条件下,ADSCs有向软骨细胞分化的潜能,为软骨组织工程种子细胞来源的研究提供了新方法。AIM： To find a method for isolation and cultivation of human adipose-derived mesenchymal stem cells （ADSCs）, and to investigate its chondrogenic ability. METHODS： Liposuction human adipose tissues were digested with collagenase type Ⅱ. The obtained cells were primarily cultured in DMEM medium, and then changed into the induced medium. Alcian Blue stain, picrosirius red stain and the specific collagenase type Ⅱ immunohistochemical staining were used to detect the results. RESULTS： After cultured in chondrogenic medium, the cells differentiated toward cartilage cells and secreted the specific cartilaginous matrices sulfated proteglycan and collagen type Ⅱ. CONCLUSION： The human ADSCs have the potential ability to differentiate toward cartilage cells in the appropriated medium. The result provide a new method for the further research in cartilaginous tissue engineering.

关 键 词：脂肪基质干细胞 软骨 诱导 

分 类 号：R318.5[医药卫生—生物医学工程]