低血清培养对骨髓间充质干细胞细胞周期同步化的影响  

Effect of low serum culture on the synchrony of cell cycle of mesenchymal stem cells

作　　者：周竹娟[1] 郑健[1] 

机构地区：[1]第三军医大学新桥医院神经内科,重庆400037

出　　处：《细胞与分子免疫学杂志》2007年第4期369-371,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30270490);重庆市科学计划项目(7452)

摘　　要：目的：探索骨髓间充质干细胞细胞周期同步在G0/G1期的培养条件.方法：培养骨髓间充质干细胞并用CD44、 CD90、 CD71、 CD11b进行流式细胞术鉴定, 观察50、 5和100 mL/L胎牛血清培养条件下细胞周期的变化情况及对细胞凋亡的影响.结果：骨髓间充质干细胞CD44、 CD90、 CD71阳性, CD11b阴性.50　mL/L胎牛血清培养1　d和5　mL/L胎牛血清培养1 ～3 d可使G0/G1期细胞比例减少, S期和G2期细胞比例增加, 但随着时间延长, G0/G1期细胞比例明显增加, S期和G2期细胞比例减少, 细胞周期阻滞在G0/G1期, 50 mL/L胎牛血清不增加细胞凋亡比例, 5 mL/L胎牛血清在3 d内使细胞凋亡比例明显增加, 随时间延长又逐渐下降.5 mL/L胎牛血清培养5 d组G0/G1期细胞比例增加最为明显, 由75.9%上升到89.4%, 凋亡细胞比例仅为0.162%.结论：5 mL/L胎牛血清培养5 d是使间充质干细胞细胞周期同步在G0/G1期的理想的培养条件.AIM： To investigate a suitable method of inducing cell cycle synchronization at G0/G1 stage of mesenchymal stem cells （MSCs）. METHODS： MSCs were cultured and identified with CD44, CD90, CD71 and CD11b by flow cytometer. Cell cycle and apotosis under normal and low serum culture were detected by flow cytometer. RESULTS： MSCs were positive for CD44, CD90 and CD71 and negative for CD11b. The cells at G0/G1 cell stage decreased, while the cells at S and G2 cell stage increased in 1 day of 50 mL/L fetal bovine serum （FBS） and 1 to 3 days of 5 mL/L FBS. However, prolonged culture in serum-starvation medium induced cell cycle arrest at G0/G1 stage. The ratio of apoptotic cells did not increase in 50 mL/L FBS. In 5 mL/L FBS, the ratio of apoptotic cells increased in 3 days and then decreased in 4 to 5 days. The proportion of cells at G0/G1 phase was significantly increased from 75. 9% to 89. 4% while the ratio of apoptotic cells was only 0. 162% in 5 days of 5 mL/L FBS. CONCLUSION： It is a good method to induce the synchrony of cell cycle of MSCs at G0/G1 stage in five days of 5 mL/L FBS.

关 键 词：骨髓间充质干细胞 细胞周期 凋亡 

分 类 号：Q253[生物学—细胞生物学]