接种白腐菌堆制法修复Pb污染土壤的研究  

Bioremediation of lead-contaminated soils by white-rot fungi as feasible inocula

作　　者：黄丹莲[1] 曾光明[1] 蒋晓云[1] 冯冲凌[1] 郁红艳[1] 陈洋[1] 刘鸿亮[1] 

机构地区：[1]湖南大学环境科学与工程学院,长沙410082

出　　处：《高技术通讯》2007年第3期307-313,共7页Chinese High Technology Letters

基　　金：国家863计划（2004AA649370）,国家973计划（No.2005CB724203）、国家杰出青年科学基金（50425927、50225926）和2000年度高等学校优秀青年教学科研奖励计划项目资助.

摘　　要：采用接种白腐菌堆制法对模拟Pb污染土壤进行了生物修复。通过监测土壤基本理化性状、土壤中Pb的各形态含量及微生物学指标（微生物生物量、微生物代谢商、微生物商、微生物C/N、微生物群落代谢能力）,系统地研究了白腐菌修复作用下土壤理化性状的动态变化、Pb生物有效性的改变以及土壤微生物学效应的变化。结果表明,接种白腐菌堆制法能有效地修复Pb污染土壤,降低Pb的生物有效性,减小Pb对土壤微生物的抑制,改善土壤中微生物效应。污染土壤经接种白腐菌堆制法修复后,土壤中Pb主要以残留态和铁锰氧化物结合态存在,水溶交换态Pb分配系数为0;微生物生物量较高,达2.258mg/g;微生物群落代谢能力较强,丰富度为72目,群落指数为4.995。The simulative lead-contaminated soils were prepared for laboratory, experiments. White-rot fungi were used as feasible inocula to bioremediate the contaminated soils. The soil samples＇ essential physico-chemical characteristics, Pb content, and biological parameters including microbial biomass, microbial metabolic quotient, microbial quotient, microbial C/N and microbial community metabolism, were all determined during the bioremediation process to study the soils＇ changes in physicochemical characteristics, bioavailability of Ph, and microbiological effects. The results showed that the white-rot fungi as the inocula led to the reduction of Pb bioavailability, the alleviation of the potential harm to soil microorganisms from Pb, and the improvement of microbiological effects. After the bioremediation by exogenous white-rot fungi, the Pb mainly existed in residual fractions and Fe-Mn oxides in the soils, and the distribution coefficient of Pb in soluble-exchangeable fraction is 0, and the microbial biomass, community richness and Shannon index reached 2. 258 mg/g, 72 and 4.995, respectively.

关 键 词：白腐菌 修复 PB污染土壤 

分 类 号：X53[环境科学与工程—环境工程]