人M5型白血病-SCID小鼠模型的建立及白血病病理变化的分析  

Establishment of human acute monocytic leukemia model in severe combined immunodeficient (SCID) mice and the analysis of pathological changes

作　　者：周斌[1] 居颂光[1] 居颂文[1] 谢芳[1] 张学光[1] 

机构地区：[1]苏州大学第一附属医院临床免疫实验室苏州大学生物技术研究所,江苏苏州215007

出　　处：《细胞与分子免疫学杂志》2007年第6期501-503,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金青年科学基金资助(30400394);江苏省135工程基金资助(2003-2006年)

摘　　要：目的:建立人M5型白血病细胞系SHI-1/SCID(重症联合免疫缺陷)小鼠模型,探讨白血病细胞的接种密度与SCID小鼠成瘤率及病理变化的关系。方法:将不同密度的人白血病细胞SHI-1注射至SCID小鼠腹腔。定期尾静脉取血并以流式细胞术(FCM)监测肿瘤细胞表面标志CD14及CD137L的变化。通过病理组织学检查和FCM分析SCID小鼠的骨髓、肝脏、脾脏和肾脏中白血病细胞的浸润及病理变化。结果:将不同密度的SHI-1细胞移植至小鼠腹腔,均可发现瘤块生长。同时中、高剂量组小鼠的主要组织器官呈现不同程度的肿瘤细胞的浸润。最早在移植3周后即可在尾静脉检测到肿瘤细胞,并与注射细胞量相关。结论:建立的SHI-1/SCID小鼠模型为人M5型白血病的研究提供了有价值的动物模型。AIM： To establish a model of human M5 leukemia and investigate the pathomorphological change in severe combined immunodeficient （SCID） mice after being transplanted with SHI-1 cells. METHODS. Various dose of SHI-1 cells were transplanted i. p. into SCID mice. Serial tail vein samples at regular intervals were prepared for detecting the expression of CD14, CD137L on SHI-1 cells by flow cytometry （FCM）. All dying mice were dissectted and the liver, spleen, kidney and bone marrow were examined to detect the appearance and distribution of the SHI-1 cells by FCM and/or immunohistochemistry. RESULTS： Tumors appeared in the abdomenion of the mice treated with various dose of SHI-1 cells. However, the dissemination of SHI-1 cells in murine tissues was found only in the groups of middle/high dose. SHI-1 cells were firstly found in tail vein samples 3 weeks after transplantation, and the time of engraftment was related to the dose of cells. CONCLUSION： The SHI-1/SCID mice was constructed, which provided a useful tool to investigate acute monocytic leukemia（AML）.

关 键 词：白血病 急性 SCID小鼠 模型 

分 类 号：R392.31[医药卫生—免疫学]